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dc.contributor.authorLau, B. Y. C.en
dc.contributor.authorDeb-Choudhury, S.en
dc.contributor.authorMorton, James D.en
dc.contributor.authorClerens, S.en
dc.contributor.authorDyer, J. M.en
dc.contributor.authorRamli, U. S.en
dc.date.accessioned2017-09-04T02:51:53Z
dc.date.available2015-12-22en
dc.date.issued2015-12-22en
dc.date.submitted2015-12-01en
dc.identifier.citationLau et al. (2015). Method developments to extract proteins from oil palm chromoplast for proteomic analysis. SpringerPlus, 4(791). doi:10.1186/s40064-015-1576-4en
dc.identifier.issn2193-1801en
dc.identifier.urihttps://hdl.handle.net/10182/8519
dc.description.abstractProteins from the plant chromoplast are essential for many physiological processes such as fatty acid biosynthesis. Different protein extraction methods were tested to find the most robust method to obtain oil palm chromoplast proteins for mass spectrometry analysis. Initially, two different solvents were employed to reduce the fruit lipids. Then, two plant cell wall digestive enzymes were used to acquire the protoplasts to increase the protein extraction effectiveness. A two-stage centrifugation-based fractionation approach enhanced the number of identified proteins, particularly the fatty acid biosynthetic enzymes. The effectiveness of each extraction method was assessed using protein yields and 2DE gel profiles. The ideal method was successfully used to establish the 2DE chromoplast proteome maps of low and high oleic acid mesocarps of oil palm. Further nanoLC–MS/MS analysis of the extracted chromoplast proteins led to the identification of 162 proteins, including some of the main enzymes involved in the fatty acid biosynthesis. The established procedures would provide a solid foundation for further functional studies, including fatty acid biosynthetic expression profiling and evaluation of regulatory function.en
dc.format.extent13en
dc.language.isoenen
dc.publisherSpringerOpenen
dc.relationThe original publication is available from - SpringerOpen - https://doi.org/10.1186/s40064-015-1576-4en
dc.relation.urihttps://doi.org/10.1186/s40064-015-1576-4en
dc.rights© 2015 Lau et al. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectElaesis guineensisen
dc.subjectchromoplasten
dc.subjectfatty acid biosynthesisen
dc.subjectprotein extractionen
dc.subjectgel electrophoresisen
dc.subjectproteomicsen
dc.subjectElaeis guineensisen
dc.titleMethod developments to extract proteins from oil palm chromoplast for proteomic analysisen
dc.typeJournal Article
lu.contributor.unitLincoln Universityen
lu.contributor.unitFaculty of Agriculture and Life Sciencesen
lu.contributor.unitDepartment of Wine, Food and Molecular Biosciencesen
lu.contributor.unitResearch Management Officeen
lu.contributor.unit/LU/Research Management Office/2018 PBRF Staff groupen
dc.identifier.doi10.1186/s40064-015-1576-4en
dc.subject.anzsrc0601 Biochemistry and Cell Biologyen
dc.subject.anzsrc060702 Plant Cell and Molecular Biologyen
dc.relation.isPartOfSpringerPlusen
pubs.issue791en
pubs.organisational-group/LU
pubs.organisational-group/LU/Agriculture and Life Sciences
pubs.organisational-group/LU/Agriculture and Life Sciences/WFMB
pubs.organisational-group/LU/Research Management Office
pubs.organisational-group/LU/Research Management Office/2018 PBRF Staff group
pubs.publication-statusPublisheden
pubs.volume4en
dc.identifier.eissn2193-1801en
dc.rights.licenceAttributionen
dc.rights.licenceAttributionen
lu.identifier.orcid0000-0001-9645-5568


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