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dc.contributor.authorHoldaway, R. J.en
dc.contributor.authorWood, J. R.en
dc.contributor.authorDickie, Ianen
dc.contributor.authorOrwin, K. H.en
dc.contributor.authorBellingham, P. J.en
dc.contributor.authorRichardson, S. J.en
dc.contributor.authorLyver, P. O.en
dc.contributor.authorTimoti, P.en
dc.contributor.authorBuckley, T. R.en
dc.date.accessioned2017-09-20T21:57:40Z
dc.date.available2017-05-16en
dc.date.issued2017en
dc.identifier.citationHoldaway et al. (2017). Using DNA metabarcoding to assess New Zealand's terrestrial biodiversity. New Zealand Journal of Ecology, 41(2), 251-262. doi:10.20417/nzjecol.41.28en
dc.identifier.issn0110-6465en
dc.identifier.urihttps://hdl.handle.net/10182/8621
dc.description.abstract© New Zealand Ecological Society. High throughput DNA sequencing technology has enabled entire biological communities to be characterised from DNA derived from pools of organisms, such as bulk-collected invertebrates, or DNA extracted from environmental samples (e.g. soil). These DNA-based techniques have the potential to revolutionise biodiversity monitoring. One approach in particular, DNA metabarcoding, can provide unprecedented taxonomic breadth at a scale not practically achievable through the morphological identification of individual organisms. Here, we assess the current strengths and weaknesses of DNA metabarcoding techniques for biodiversity assessment. We argue that it is essential to integrate conventional monitoring methods with novel DNA methods, to validate methods, and to better use and interpret data. We present a conceptual framework for how this might be done, explore potential applications within national biodiversity assessment frameworks, Maori biodiversity monitoring and the primary sector, and highlight areas of current uncertainty and future research directions. Rapid developments in DNA sequencing technology and bioinformatics will make DNA-based community data increasingly accessible to ecologists, and there needs to be a corresponding shift in research focus from DNA metabarcoding method development and evaluation to real-world applications that provide rich information for a range of purposes, including conservation planning and land management decisions.en
dc.format.extent251-262en
dc.language.isoenen
dc.publisherNew Zealand Ecological Societyen
dc.relationThe original publication is available from - New Zealand Ecological Society - https://doi.org/10.20417/nzjecol.41.28en
dc.relation.urihttps://doi.org/10.20417/nzjecol.41.28en
dc.rights© New Zealand Ecological Societyen
dc.subjectbiodiversity monitoringen
dc.subjectbioinformaticsen
dc.subjectbiosecurityen
dc.subjectcultural indicatorsen
dc.subjectecosystem functionen
dc.subjectenvironmental DNAen
dc.subjectnational frameworken
dc.subjectspecies detectionen
dc.subjectte ao Maorien
dc.subjectuncertaintyen
dc.subjectEcologyen
dc.titleUsing DNA metabarcoding to assess New Zealand’s terrestrial biodiversityen
dc.typeJournal Article
lu.contributor.unitLincoln Universityen
lu.contributor.unitBio-Protection Research Centreen
dc.identifier.doi10.20417/nzjecol.41.28en
dc.subject.anzsrc050202 Conservation and Biodiversityen
dc.subject.anzsrc0602 Ecologyen
dc.relation.isPartOfNew Zealand Journal of Ecologyen
pubs.issue2en
pubs.organisational-group/LU
pubs.organisational-group/LU/BPRC
pubs.publication-statusPublisheden
pubs.volume41en
lu.identifier.orcid0000-0002-2740-2128


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