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dc.contributor.authorXu, Boyuen
dc.date.accessioned2009-02-25T21:04:26Z
dc.date.issued2008en
dc.identifier.urihttps://hdl.handle.net/10182/864
dc.description.abstractThe administration of DNA vaccine has been suggested as a promising method to increase specific humoral and cellular immune response against Toxoplasma gondii (T. gondii) infection. In the current study, the serological (immunoglubins IgG1 and IgG2) responses of sheep to DNA vaccination against T. gondii were evaluated. The cloned plasmid DNA containing protective T. gondii antigens, ROP1, GRA7, MIC3, GRA1, GRA6 and MAG1 were transformed and grown in Escherichia coli cells and purified. Three groups of coopworth twelve month old ewes (n=40) were immunised by intramuscular injection with the combination of purified plasmids pVAXROP1/CD154+pVAXIgGRA7+pVAXMIC3 and/or pVAXIgGRA1+pVAXIgGRA6+pVAXMAG1 separately, as well as plasmid encoding CpG oligodeoxynucleotide and liposomes as adjuvants. Another group of ewes (n=40) were injected with phosphate buffered saline solution as controls. The injections were applied twice intramuscularly with a four weeks interval between injections. Production of IgG1 and IgG2 antibodies were studied by the use of enzyme linked immunosorbent assays, with the optical density ratio representing antibody titre response. The first and second immunisations with the combination of plasmids pVAXROP1/CD154+pVAXIgGRA7+pVAXMIC3 produced high (P<0.001) level of specific anti-ROP1 IgG1 antibody in the serum. This indicates that this technique could stimulate strong humoral immune response, and therefore has promise for development of a commercial Toxoplasma vaccine.en
dc.formatx, 65 leaves
dc.language.isoenen
dc.publisherLincoln Universityen
dc.subjectToxoplasma gondiien
dc.subjectsheepen
dc.subjectDNA vaccineen
dc.subjectantibodyen
dc.subjecttoxoplasmosisen
dc.titleThe serological response of sheep to DNA immunisation against Toxoplasma gondii : a dissertation submitted in partial fulfilment of the requirements for the degree of Master of Applied Science at Lincoln Universityen
dc.typeThesis
thesis.degree.grantorLincoln Universityen
thesis.degree.levelMastersen
thesis.degree.nameMaster of Applied Scienceen
dc.subject.marsdenFields of Research::300000 Agricultural, Veterinary and Environmental Sciences::300500 Veterinary Medicine::300508 Parasitologyen
dc.subject.marsdenFields of Research::300000 Agricultural, Veterinary and Environmental Sciences::300500 Veterinary Medicine::300504 Immunologyen
lu.contributor.unitLincoln Universityen
lu.contributor.unitFaculty of Agriculture and Life Sciencesen
lu.contributor.unitDepartment of Wine, Food and Molecular Biosciencesen
dc.rights.accessRightsDigital dissertation can be viewed by current staff and students of Lincoln University only.
pubs.organisational-group/LU
pubs.organisational-group/LU/Agriculture and Life Sciences
pubs.organisational-group/LU/Agriculture and Life Sciences/WFMB
pubs.publication-statusPublisheden


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