Research@Lincoln
    • Login
     
    View Item 
    •   Research@Lincoln Home
    • Metadata-only (no full-text)
    • Metadata-only (no full-text)
    • View Item
    •   Research@Lincoln Home
    • Metadata-only (no full-text)
    • Metadata-only (no full-text)
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Haplotyping using a combination of polymerase chain reaction–single-strand conformational polymorphism analysis and haplotype-specific PCR amplification

    Zhou, Huitong; Li, S.; Liu, X.; Wang, J.; Luo, Y.; Hickford, Jonathan G. H.
    Abstract
    A single nucleotide polymorphism (SNP) may have an impact on phenotype, but it may also be influenced by multiple SNPs within a gene; hence, the haplotype or phase of multiple SNPs needs to be known. Various methods for haplotyping SNPs have been proposed, but a simple and cost-effective method is currently unavailable. Here we describe a haplotyping approach using two simple techniques: polymerase chain reaction–single-strand conformational polymorphism (PCR–SSCP) and haplotype-specific PCR. In this approach, individual regions of a gene are analyzed by PCR–SSCP to identify variation that defines sub-haplotypes, and then extended haplotypes are assembled from the sub-haplotypes either directly or with the additional use of haplotype-specific PCR amplification. We demonstrate the utility of this approach by haplotyping ovine FABP4 across two variable regions that contain seven SNPs and one indel. The simplicity of this approach makes it suitable for large-scale studies and/or diagnostic screening.... [Show full abstract]
    Keywords
    haplotyping; SNP haplotype; PCR–SSCP; haplotype-specific PCR amplification; PCR-SSCP; Biochemistry & Molecular Biology; Animals; Sheep; Humans; DNA Fingerprinting; Nucleic Acid Amplification Techniques; Polymerase Chain Reaction; Base Sequence; Haplotypes; Polymorphism, Single-Stranded Conformational; Molecular Sequence Data; Fatty Acid-Binding Proteins
    Fields of Research
    060101 Analytical Biochemistry
    Date
    2014-12-01
    Type
    Journal Article
    Collections
    • Metadata-only (no full-text) [4903]
    View/Open
    Share this

    on Twitter on Facebook on LinkedIn on Reddit on Tumblr by Email

    DOI
    https://doi.org/10.1016/j.ab.2014.08.021
    Metadata
     Expand record
    © 2014 Elsevier Inc. All rights reserved.
    Citation
    Zhou et al. (2014). Haplotyping using a combination of polymerase chain reaction–single-strand conformational polymorphism analysis and haplotype-specific PCR amplification. Analytical Biochemistry, 466, 59-64. doi:10.1016/j.ab.2014.08.021

    Related items

    Showing items related by title, author, creator and subject.

    • Using molecules to identify the source of fruit fly invasions 

      Barr, N.; Ruiz-Arce, R.; Armstrong, Karen F. (SpringerNew York, 2014-07-29)
      Fruit flies trapped or intercepted as part of inspection and surveillance activities could be the result of resurgent pest populations, incursions from offshore introductions, or intentional releases of sterile flies. ...
    • Haplotypic variation in the UCP1 gene is associated with milk traits in dairy cows 

      Zhou, Huitong; Cheng, Paul; Byun, Seung O.; Aizimu, W.; Hodge, Miriam; Edwards, Grant; Hickford, Jonathan G. H. (American Dairy Science Association, 2016-10-03)
      Uncoupling protein-1 (UCP1) plays a role in the regulation of body temperature, metabolic rate and energy expenditure in animals. While variation in UCP1 and its phenotypic effect has been investigated in humans and sheep, ...
    • Conservation and distribution of leopard (Panthera pardus) in northern Pakistan : A thesis submitted in partial fulfilment of the requirements for the Degree of Doctor of Philosophy at Lincoln University 

      Asad, Muhammad (Lincoln University, 2020)
      Common leopard (Panthera pardus Linnaeus, 1758) populations are in serious decline throughout the range of the species. The leopard is considered a vulnerable species globally and is critically endangered in Pakistan, ...
    This service is maintained by Learning, Teaching and Library
    • Archive Policy
    • Copyright and Reuse
    • Deposit Guidelines and FAQ
    • Contact Us
     

     

    Browse

    All of Research@LincolnCommunities & CollectionsTitlesAuthorsKeywordsBy Issue DateThis CollectionTitlesAuthorsKeywordsBy Issue Date

    My Account

    LoginRegister

    Statistics

    View Usage Statistics
    This service is maintained by Learning, Teaching and Library
    • Archive Policy
    • Copyright and Reuse
    • Deposit Guidelines and FAQ
    • Contact Us