Use of Coniothyrium minitans transformed with the hygromycin B resistance gene to study survival and infection of Sclerotinia sclerotiorum sclerotia in soil

dc.contributor.authorJones, Elizabeth
dc.contributor.authorStewart, A
dc.contributor.authorWhipps, J
dc.coverage.spatialEngland
dc.date.accessioned2008-03-25T20:43:39Z
dc.date.issued2003-03
dc.description.abstractA Coniothyrium minitans strain (T3) co-transformed with the genes for β-glucuronidase (uidA) and hygromycin phosphotransferase (hph), the latter providing resistance to the antibiotic hygromycin B, was used to investigate the survival and infection of sclerotia of Sclerotinia sclerotiorum by C. minitans over time in four different soils. Infection of sclerotia was rapid in all cases, with the behaviour of transformant T3 and wild type parent A69 being similar. Differences were seen between the soils in the rate of infection of sclerotia by C. minitans and in their indigenous fungal populations. Amendment of agar with hygromycin B enabled the quantification of C. minitans in soil by dilution plating where there was a high background of other microorganisms. In Lincoln soil from New Zealand, which had a natural but low population of C. minitans, the hygromycin B resistance marker allowed the umambiguous discrimination of the applied transformed isolate from the indigenous hygromycin B sensitive one. In this soil, although the indigenous C. minitans population was detected from sclerotia, none were recovered on the dilution plates, indicating the increased sensitivity of C. minitans detection from soil using sclerotial baiting. C. minitans was a very efficient parasite, being able to infect a large proportion of sclerotia within a relatively short time from an initially low soil population. The addition of hygromycin B to agar also allowed the detection of C. minitans from decaying sclerotia by inhibiting secondary fungal colonisers. This is the first report to show that fungi colonising sclerotia already infected by C. minitans mask the detection of C. minitans from sclerotia rather than displacing the original parasite.
dc.format.extentpp.267-276
dc.format.mediumPrint
dc.identifierhttps://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=elements_prod&SrcAuth=WosAPI&KeyUT=WOS:000183527200005&DestLinkType=FullRecord&DestApp=WOS
dc.identifier.citationJones, E. E., Stewart, A., & Whipps, J. M. (2003). Use of Coniothyrium minitans transformed with the hygromycin B resistance gene to study survival and infection of Sclerotinia sclerotiorum sclerotia in soil. Mycological Research, 107(3), 267-276.
dc.identifier.doi10.1017/S0953756203007457
dc.identifier.eissn1469-8102
dc.identifier.issn0953-7562
dc.identifier.other12825495 (pubmed)
dc.identifier.urihttps://hdl.handle.net/10182/373
dc.language.isoen
dc.publisherCambridge University Press
dc.relationThe original publication is available from Cambridge University Press - https://doi.org/10.1017/S0953756203007457 - http://dx.doi.org/10.1017/s0953756203007457
dc.relation.isPartOfMycological Research
dc.relation.urihttps://doi.org/10.1017/S0953756203007457
dc.rightsCopyright © The British Mycological Society 2003
dc.subjectsclerotia
dc.subjectinfection
dc.subjectConiothyrium minitans
dc.subjectSclerotinia sclerotiorum
dc.subjectsoil fungi
dc.subjectsoil mycology
dc.subject.anzsrc2020ANZSRC::3107 Microbiology
dc.subject.anzsrc2020ANZSRC::3108 Plant biology
dc.subject.marsdenMarsden::270305 Mycology
dc.subject.meshAscomycota
dc.subject.meshGlucuronidase
dc.subject.meshPhosphotransferases (Alcohol Group Acceptor)
dc.subject.meshHygromycin B
dc.subject.meshCulture Media
dc.subject.meshColony Count, Microbial
dc.subject.meshSoil Microbiology
dc.subject.meshDrug Resistance, Fungal
dc.subject.meshTransformation, Genetic
dc.titleUse of Coniothyrium minitans transformed with the hygromycin B resistance gene to study survival and infection of Sclerotinia sclerotiorum sclerotia in soil
dc.typeJournal Article
lu.contributor.unitLU
lu.contributor.unitLU|Agriculture and Life Sciences
lu.contributor.unitLU|Agriculture and Life Sciences|ECOL
lu.contributor.unitLU|OLD BPRC
lu.contributor.unitLU|Research Management Office
lu.contributor.unitLU|Research Management Office|OLD QE18
lu.contributor.unitLU|Research Management Office|OLD PE20
lu.contributor.unitLU|Centre of Excellence for One Biosecurity Research, Analysis and Synthesis
lu.identifier.orcid0000-0002-1879-4537
pubs.issue3
pubs.publication-statusPublished
pubs.publisher-urlhttp://dx.doi.org/10.1017/s0953756203007457
pubs.volume107
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