Thermal adaptation of soil microbial growth traits in response to chronic warming

dc.contributor.authorEng, AY
dc.contributor.authorNarayanan, A
dc.contributor.authorAlster, Charlotte
dc.contributor.authorDeAngelis, KM
dc.contributor.editorSpear, JR
dc.coverage.spatialUnited States
dc.date.accessioned2024-04-02T22:31:01Z
dc.date.available2023-10-25
dc.date.available2024-04-02T22:31:01Z
dc.date.issued2023-11
dc.date.submitted2023-08-31
dc.description.abstractAdaptation of soil microbes due to warming from climate change has been observed, but it remains unknown what microbial growth traits are adaptive to warming. We studied bacterial isolates from the Harvard Forest Long-Term Ecological Research site, where field soils have been experimentally heated to 5°C above ambient temperature with unheated controls for 30 years. We hypothesized that Alphaproteobacteria from warmed plots have (i) less temperature-sensitive growth rates; (ii) higher optimum growth temperatures; and (iii) higher maximum growth temperatures compared to isolates from control plots. We made high-throughput measurements of bacterial growth in liquid cultures over time and across temperatures from 22°C to 37°C in 2–3°C increments. We estimated growth rates by fitting Gompertz models to the growth data. Temperature sensitivity of growth rate, optimum growth temperature, and maximum growth temperature were estimated by the Ratkowsky 1983 model and a modified Macromolecular Rate Theory (MMRT) model. To determine evidence of adaptation, we ran phylogenetic generalized least squares tests on isolates from warmed and control soils. Our results showed evidence of adaptation of higher optimum growth temperature of bacterial isolates from heated soils. However, we observed no evidence of adaptation of temperature sensitivity of growth and maximum growth temperature. Our project begins to capture the shape of the temperature response curves, but illustrates that the relationship between growth and temperature is complex and cannot be limited to a single point in the biokinetic range.
dc.format.extent15 pages
dc.format.mediumPrint-Electronic
dc.identifierhttps://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=elements_prod&SrcAuth=WosAPI&KeyUT=WOS:001138989000006&DestLinkType=FullRecord&DestApp=WOS_CPL
dc.identifier.doi10.1128/aem.00825-23
dc.identifier.eissn1098-5336
dc.identifier.issn0099-2240
dc.identifier.other37877729 (pubmed)
dc.identifier.urihttps://hdl.handle.net/10182/17045
dc.language.isoen
dc.publisherAmerican Society for Microbiology
dc.relationThe original publication is available from American Society for Microbiology - https://doi.org/10.1128/aem.00825-23 - http://dx.doi.org/10.1128/aem.00825-23
dc.relation.isPartOfApplied and Environmental Microbiology
dc.relation.urihttps://doi.org/10.1128/aem.00825-23
dc.rights© 2023 Eng et al.
dc.rights.ccnameAttribution
dc.rights.ccurihttps://creativecommons.org/licenses/by/4.0/
dc.subjectsoil
dc.subjectsoil warming
dc.subjectclimate change
dc.subjectmicrobial evolution
dc.subjectmicrobiol ecology
dc.subject.anzsrc2020ANZSRC::410603 Soil biology
dc.subject.anzsrc2020ANZSRC::310703 Microbial ecology
dc.subject.anzsrc2020ANZSRC::410102 Ecological impacts of climate change and ecological adaptation
dc.subject.anzsrc2020ANZSRC::3107 Microbiology
dc.subject.anzsrc2020ANZSRC::3207 Medical microbiology
dc.subject.meshSoil
dc.subject.meshSoil Microbiology
dc.subject.meshBiomass
dc.subject.meshClimate Change
dc.subject.meshGlobal Warming
dc.titleThermal adaptation of soil microbial growth traits in response to chronic warming
dc.typeJournal Article
lu.contributor.unitLU
lu.contributor.unitLU|Agriculture and Life Sciences
lu.contributor.unitLU|Agriculture and Life Sciences|SOILS
lu.identifier.orcid0000-0001-9257-771X
pubs.issue11
pubs.publication-statusPublished
pubs.publisher-urlhttp://dx.doi.org/10.1128/aem.00825-23
pubs.volume89
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