Identification of the ovine KAP11-1 gene (KRTAP11-1) and genetic variation in its coding sequence

dc.contributor.authorGong, H
dc.contributor.authorZhou, Huitong
dc.contributor.authorDyer, J
dc.contributor.authorHickford, Jonathan
dc.coverage.spatialNetherlands
dc.date.accessioned2016-02-19T00:51:04Z
dc.date.available2011-03-12
dc.date.issued2011
dc.date.submitted2011-02-26
dc.description.abstractKeratin-associated proteins (KAPs) are a structural component of the wool fibre and form the matrix between the keratin intermediate filaments (KIFs). The gene encoding high sulphur-protein KAP11-1 has been identified in human, cattle and mouse, but not yet in sheep, despite the economic importance of wool. In this study, PCR using primers based on the cattle KAP11-1 gene sequence produced an amplicon of the expected size with sheep DNA. Upon using PCR-Single Stranded Conformational Polymorphism (PCR-SSCP) analysis in 260 sheep, six different PCR-SSCP patterns were detected. Either one or a combination of two banding patterns was observed for each sheep, suggesting they were either homozygous or heterozygous for this gene. Sequencing of the amplicons confirmed the occurrence of six DNA sequences. All of these were unique, and the greatest homology was with KRTAP11-1 sequences from cattle, human and mouse, suggesting that they were derived from the ovine KAP11-1 gene and were allelic variants. The ovine KAP11-1 gene had an open reading frame of 477 nucleotides encoding 159 amino acids. The putative protein was rich in serine, cysteine, and threonine which account for 18.2-18.9, 12.6 and 12.0 mol%, respectively. Of these, approximately 20 of the serine and threonine residues might be phosphorylated. Five nucleotide substitutions were identified, and one was non-synonymous and would result in an amino acid change at a potential phosphorylation site. The genetic variation found in KRTAP11-1 may influence its expression, protein structure, and/or posttranslational modifications, and consequently affect wool fibre structure and wool traits. © 2011 Springer Science+Business Media B.V.
dc.format.extentpp.5429-5433
dc.format.mediumPrint-Electronic
dc.identifierhttps://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=elements_prod&SrcAuth=WosAPI&KeyUT=WOS:000296013600074&DestLinkType=FullRecord&DestApp=WOS
dc.identifier.citationGong, H., Zhou, H., Dyer, J.M. & Hickford, JG.H. (2011). Identification of the ovine KAP11-1 gene (KRTAP11-1) and genetic variation in its coding sequence. Molecular Biology Reports, 38(8), 5429-5433. DOI: 10.1007/s11033-011-0697-2
dc.identifier.doi10.1007/s11033-011-0697-2
dc.identifier.eissn1573-4978
dc.identifier.issn0301-4851
dc.identifier.other21400094 (pubmed)
dc.identifier.urihttps://hdl.handle.net/10182/6861
dc.languageen
dc.language.isoen
dc.publisherSpringer
dc.relationThe original publication is available from Springer - https://doi.org/10.1007/s11033-011-0697-2 - http://dx.doi.org/10.1007/s11033-011-0697-2
dc.relation.isPartOfMolecular Biology Reports
dc.relation.urihttps://doi.org/10.1007/s11033-011-0697-2
dc.rightsCopyright © Springer Science+Business Media B.V. 2011
dc.subjectKeratin-associated protein KAP11-1 gene (KRTAP11-1)
dc.subjectVariation
dc.subjectPhosphorylation
dc.subjectPCR-SSCP
dc.subjectSheep
dc.subject.anzsrc2020ANZSRC::3101 Biochemistry and cell biology
dc.subject.meshAnimals
dc.subject.meshSheep, Domestic
dc.subject.meshHumans
dc.subject.meshAmino Acid Substitution
dc.subject.meshPolymerase Chain Reaction
dc.subject.meshSequence Alignment
dc.subject.meshBreeding
dc.subject.meshAmino Acid Sequence
dc.subject.meshBase Sequence
dc.subject.meshGene Frequency
dc.subject.meshPolymorphism, Single-Stranded Conformational
dc.subject.meshOpen Reading Frames
dc.subject.meshMolecular Sequence Data
dc.subject.meshKeratins
dc.subject.meshGenetic Variation
dc.titleIdentification of the ovine KAP11-1 gene (KRTAP11-1) and genetic variation in its coding sequence
dc.typeJournal Article
lu.contributor.unitLU
lu.contributor.unitLU|Agriculture and Life Sciences
lu.contributor.unitLU|Agriculture and Life Sciences|AGSC
lu.contributor.unitLU|Research Management Office
lu.contributor.unitLU|Research Management Office|OLD QE18
lu.contributor.unitLU|Centre of Excellence for One Biosecurity Research, Analysis and Synthesis
lu.identifier.orcid0000-0001-7620-3160
lu.identifier.orcid0000-0001-9313-761X
pubs.issue8
pubs.publication-statusPublished
pubs.publisher-urlhttp://dx.doi.org/10.1007/s11033-011-0697-2
pubs.volume38
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