Pan, HFeng, HLiu, YLai, C-YZhuge, YZhang, QTang, CDi, HongJia, ZGubry-Rangin, CLi, YXu, J2024-08-142021-12-092024-08-142021-122021-10-182730-615136765259 (pubmed)PMC9723554 (pmc)https://hdl.handle.net/10182/17446Grassland soils serve as a biological sink and source of the potent greenhouse gases (GHG) methane (CH₄) and nitrous oxide (N₂O). The underlying mechanisms responsible for those GHG emissions, specifically, the relationships between methane- and ammonia-oxidizing microorganisms in grazed grassland soils are still poorly understood. Here, we characterized the effects of grazing on in situ GHG emissions and elucidated the putative relations between the active microbes involving in methane oxidation and nitrification activity in grassland soils. Grazing significantly decreases CH₄ uptake while it increases N₂O emissions basing on 14-month in situ measurement. DNA-based stable isotope probing (SIP) incubation experiment shows that grazing decreases both methane oxidation and nitrification processes and decreases the diversity of active methanotrophs and nitrifiers, and subsequently weakens the putative competition between active methanotrophs and nitrifiers in grassland soils. These results constitute a major advance in our understanding of putative relationships between methane- and ammonia-oxidizing microorganisms and subsequent effects on nitrification and methane oxidation, which contribute to a better prediction and modeling of future balance of GHG emissions and active microbial communities in grazed grassland ecosystems.12 pagesElectronicen© The Author(s) 2021, corrected publication 2022Journal Article10.1038/s43705-021-00068-22730-6151ANZSRC::370203 Greenhouse gas inventories and fluxesANZSRC::410601 Land capability and soil productivityANZSRC::410604 Soil chemistry and soil carbon sequestration (excl. carbon sequestration science)ANZSRC::310703 Microbial ecologyANZSRC::3103 EcologyANZSRC::3107 Microbiologyhttps://creativecommons.org/licenses/by/4.0/Attribution