Oledzka, GLi, BKay, GChomicz, LStankiewicz, M2008-03-312006-06-1320072006-04-10Oledzka, G., Li, B., Kay, G. W., Chomicz, L., & Stankiewicz, M. (2007). Cloning, sequence analysis and expression of ovine CD154 (CD40 ligand). Molecular Immunology, 44(5), 741-746.0161-589016777229 (pubmed)https://hdl.handle.net/10182/408The CD154 (CD40 ligand) molecule is a member of the tumor necrosis factor (TNF) family and plays an important role in the interaction between antigen-specific lymphocytes and antigen-presenting cells. In this study, reverse transcription-PCR cloning was used to derive the sequence encoding ovine CD154. Sequence analysis of the cloned CD154 gene showed a similarity of 97%, 89%, and 88% with the bovine, porcine and human sequences, respectively, at the nucleic acid level. The deduced amino acid sequence for the ovine CD154 shared 97%, 91%, and 87% similarity with the CD154 protein of bovine, porcine and human. The cysteine residues characteristic of the TNF family and N-linked glycosylation sites are conserved although one of the cysteine residues (Cys9) appeared only in ovine CD154. The isolated CD154 sequence was expressed as a mature protein in Chinese hamster ovary (CHO) cells. The analysis of expression of ovine CD154 in mammalian cells by Western blot confirmed the cross reactivity with anti-CD154 antibody.pp.741-746Print-ElectronicenCopyright © 2006 Elsevier Ltd All rights reservedsheepCD154CD40 ligandDNA vaccineCHO CellsAnimalsSheepCricetulusCD40 LigandCloning, MolecularSequence Analysis, DNAAmino Acid SequenceBase SequenceSequence Homology, Amino AcidMolecular Sequence DataCricetinaeJournal ArticleMarsden::270206 Genetic immunology10.1016/j.molimm.2006.04.0121872-9142ANZSRC::3101 Biochemistry and cell biologyANZSRC::3204 Immunology