Fang, Haitian,Al-Marashdeh, OmarZhou, HuitongPodolyan, AndriyHickford, JonathanEdwards, GRCameron, KeithCheng, L2018-11-182018-07-092018-07-092018-06-26Fang et al. (2018). Ex-vivo cow rumen fluid fermentation: Changes in microbial populations and fermentation products with different forages. Journal of Applied Animal Research, 46(1), 1272-1279. doi:10.1080/09712119.2018.14950820971-2119GM4YI (isidoc)https://hdl.handle.net/10182/10358This study investigated the effects of three forage treatments [herbal plantain (PL), ryegrass-white clover pasture (RW) and root crop fodder beet (FB)] on ex-vivo rumen fluid fermentations. Quantitative real-time Polymerase Chain Reaction (qPCR) and PCR-single-stranded conformation polymorphism (PCR-SSCP) analyses were undertaken for aliquots collected from the fermentations. The FB treatment had the highest volatile fatty acid (VFA) and lowest pH compared with other treatments. The RW culture that had a higher fibre content, had higher Fibrobacter succinogenes(F. succinogenes) and anaerobic fungi levels when compared with the FB and PL. Protozoans were however the most abundant microorganisms in the FB cultures, and they had the highest water-soluble carbohydrate (WSC) content. The abundance of F. succinogenes increased in the RW fermentation up to 12 h, and the abundance of anaerobic fungi increased in the RW fermentation up to 24 h. In all the treatments, no effect was found on the overall abundance of bacteria. The findings confirmed that changes in rumen microbial community and fermentation products are partly related to the WSC and fibre content of two novel forages (i.e. PL and FB) increasingly used in animal production.pp.1272-1279en© 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Groupfodder beetplantainpasturemicrobial populationVFAqPCRSSCPEx-vivo cow rumen fluid fermentation: Changes in microbial populations and fermentation products with different foragesJournal Article10.1080/09712119.2018.1495082ANZSRC::070204 Animal Nutrition0974-1844ANZSRC::3003 Animal productionANZSRC::3009 Veterinary sciencesANZSRC::3109 Zoologyhttps://creativecommons.org/licenses/by/4.0/Attribution