Najafabadi, HAKhansefid, MMahmoud, GGHaruna, ILZhou, HuitongHickford, Jonathan2021-09-082021-08-112021-092021-08-030301-485134379287 (pubmed)https://hdl.handle.net/10182/14195Advances in the study of reproductive traits indicate that functional variation in fertility genes may be useful for improving sheep fertility. The aim of this study was to search for variation in the bone morphogenetic protein 15 gene (BMP15) and ascertain any association with litter size in purebred Finnish Landrace sheep (n = 148), Finnish Landrace × Texel-cross sheep (n = 45), and composite sheep (of varying breed background; n = 58) from New Zealand (NZ). A 482 bp and 312 bp fragment of exon 1 and 2, respectively, of BMP15 were analysed using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP). The additive and dominance effect of BMP15 variation on litter size were estimated using animal and sire models. Two variants (A and B) were detected in exon 1; no sequence variation was detected in exon 2. Variant A had the nucleotide sequence CTT between positions c.31 and c.33, while variant B had a deletion (c.31_33del). The observed frequency for variant A in the Finnish Landrace sheep, Finnish Landrace × Texel-cross sheep and the composite sheep, was 0.77, 0.92, and 0.68, respectively while the frequency of variant B (c.31_33del) was 0.23, 0.08, and 0.32, respectively. An association between litter size and c.31_33del (P < 0.001) was observed in composite sheep. Analysis of more sheep will be required to confirm these results. Litter size did not differ significantly between sheep breeds regardless of the presence/absence of c.31_33del. Results suggested that c.31_33del might be a genetic marker for improving fecundity in some NZ sheep.pp.6335-6342Print-Electronicen© The Author(s), under exclusive licence to Springer Nature B.V. 2021bone morphogenic protein 15 genelitter sizeNew ZealandOvis ariessheepOocytesAnimalsSheep, DomesticPolymerase Chain ReactionSequence Analysis, DNABase SequenceReproductionFertilityPregnancyLitter SizeHeterozygoteHomozygoteMutationPolymorphism, GeneticAllelesExonsNew ZealandFemaleBone Morphogenetic Protein 15Journal Article10.1007/s11033-021-06627-z1573-4978ANZSRC::3101 Biochemistry and cell biology