Purushotham, NJones, ElizabethMonk, JRidgway, H2020-02-242020-01-272020-01-272020-01-232223-7747KT8FU (isidoc)32012657 (pubmed)https://hdl.handle.net/10182/11453Although the importance of the plant microbiome in commercial plant health has been well established, there are limited studies in native medicinal plants. Pseudowintera colorata (horopito) is a native New Zealand medicinal plant recognized for its antimicrobial properties. Denaturing gradient gel electrophoresis (DGGE) and Illumina MiSeq analysis of P. colorata plants from ten sites across New Zealand showed that tissue type strongly influenced the diversity and richness of endophytic bacteria (PERMANOVA, P < 0.05). In addition, two OTUs belonging to the genus Pseudomonas (Greengenes ID: 646549 and 138914) were found to be present in >75% of all P. colorata leaf, stem and root samples and were identified as the members of the P. colorata “core endomicrobiome”. Culture-independent analysis was complemented by the recovery of 405 endophytic bacteria from the tissues of P. colorata. Some of these cultured endophytic bacteria (n = 10) showed high antagonism against four different phytopathogenic fungi tested. The influence of endophytic bacteria on plant growth was assessed by inoculating P. colorata seedlings. The mean shoot height of seedlings treated with Bacillus sp. TP1LA1B were longer (1.83×), had higher shoot dry weight (1.8×) and produced more internodes (1.8×) compared to the control.14 pagesElectronicen© 2020 by the authors. Licensee MDPI, Basel, Switzerland.endophytic bacteriaendophytesplant-microbe interactionsplant growth promotionmicrobial ecologymedicinal plantIllumina MiSeqJournal Article10.3390/plants9020156ANZSRC::0607 Plant BiologyANZSRC::0605 MicrobiologyANZSRC::060501 Bacteriology2223-7747ANZSRC::30 Agricultural, veterinary and food sciencesANZSRC::31 Biological scienceshttps://creativecommons.org/licenses/by/4.0/Attribution