Deng, QPu, YSun, LWang, YalingLiu, YangWang, RLiao, JXu, DLiu, YYe, RiyingFang, ZGooneratne, R2018-08-302017-09-302017-122017-09-21Deng et al. (2017). Antimicrobial peptide AMPNT-6 from Bacillus subtilis inhibits biofilm formation by Shewanella putrefaciens and disrupts its preformed biofilms on both abiotic and shrimp shell surfaces. Food Research International, 102, 8-13. doi:10.1016/j.foodres.2017.09.0570963-996929196015 (pubmed)https://hdl.handle.net/10182/10191Shewanella putrefaciens biofilm formation is of great concern for the shrimp industry because it adheres easily to food and food-contact surfaces and is a source of persistent and unseen contamination that causes shrimp spoilage and economic losses to the shrimp industry. Different concentrations of an antimicrobial lipopeptide, the fermentation product of Bacillus subtilis, AMPNT-6, were tested for the ability to reduce adhesion and disrupt S. putrefaciens preformed biofilms on two different contact surfaces (shrimp shell, stainless steel sheet). AMPNT-6 displayed a marked dose- and time-dependent anti-adhesive effect > biofilm removal. 3MIC AMPNT-6 was able both to remove biofilm and prevent bacteria from forming biofilm in a 96-well polystyrene microplate used as the model surface. 2MIC AMPNT-6 prevented bacteria from adhering to the microplate surface to form biofilm for 3 h and removed already existing biofilm within 24 h. Secretion of extracellular polymeric substances incubated in LB broth for 24 h by S. putrefaciens was minimal at 3 × MIC AMPNT-6. Scanning electron microscopy showed that damage to S. putrefaciens bacteria by AMPNT-6 possibly contributed to the non-adherence to the surfaces. Disruption of the mature biofilm structure by AMPNT-6 contributed to biofilm removal. It is concluded that AMPNT-6 can be used effectively to prevent attachment and also detach S. putrefaciens biofilms from shrimp shells, stainless steel sheets and polystyrene surfaces.pp.8-13Print-Electronicen© 2017 Elsevier Ltd. All rights reserved.Bacillus subtilisantimicrobial lipopeptideShewanella putrefaciensanti-adhesionbiofilm removalAnimalsPenaeidaeBiofilmsBacillus subtilisShewanella putrefaciensStainless SteelPolystyrenesAnti-Bacterial AgentsMicroscopy, Electron, ScanningFood MicrobiologyBacterial AdhesionDose-Response Relationship, DrugSurface PropertiesTime FactorsFood PreservationSeafoodMicrobial ViabilityAnimal ShellsJournal Article10.1016/j.foodres.2017.09.057ANZSRC::0605 MicrobiologyANZSRC::070707 Veterinary Microbiology (excl. Virology)1873-7145ANZSRC::3006 Food sciencesANZSRC::3210 Nutrition and dieteticsANZSRC::4004 Chemical engineering