Alwarsamy, MGooneratne, SRRavichandran, R2016-10-072016-07-072016-112016-06-29Alwarsamy, M.; Gooneratne, R.; Ravichandran, R.(2016) Effect of fucoidan from Turbinaria conoides on human lung adenocarcinoma epithelial (A549) cells. Carbohydrate Polymers, 152, 207-213. doi:10.1016/j.carbpol.2016.06.1120144-861727516266 (pubmed)https://hdl.handle.net/10182/7449Fucoidan was purified from seaweed, Turbinaria conoides. Isolated fragments were characterized with NMR (13C, 1H), Gas Chromatography–Mass Spectronomy (GC–MS) and HPLC analysis. The autohydrolysate of fucoidans consisted of sulfated fuco-oligosaccharides having the backbone of α-(1, 3)-linked fuco-pyranose derivatives and minor components of galactose, glucose, mannose and xylose sugars. Fucoidan induced a dose-dependent reduction in cell survival of lung cancer A549 cells by MTT assay (GI50, 75 μg/mL). However, it was not cytotoxic to a non-tumorigenic human keratinocyte cell line of skin tissue (HaCaT) (GI50 > 1.0 mg/mL). The apoptotic cells in fucoidan-treated A549 cells were visualized by laser confocal microscopy and cell cycle analysis showed induction of G0/G1 phase arrest of the cell progression cycle. Further, CFSE labeling and flow cytometry highlighted that fucoidan significantly (P < 0.05) inhibited the proliferation rate of A549 cells by up to 2-fold compared with the control cells. It is concluded that fucoidan has the potential to act as an anti-proliferative agent on lung carcinoma (A549) cells.pp.207-213Print-Electronicen© 2016 Published by Elsevier Ltd.G0/G1 arrestapoptosisCFSE labelingstructural analysisRespiratory MucosaEpithelial CellsHumansSeaweedAdenocarcinomaLung NeoplasmsPolysaccharidesAntineoplastic AgentsDrug Screening Assays, AntitumorApoptosisCell ProliferationCell SurvivalA549 CellsAdenocarcinoma of LungPhaeophyceaeJournal Article10.1016/j.carbpol.2016.06.1121879-1344ANZSRC::3006 Food sciencesANZSRC::3101 Biochemistry and cell biologyANZSRC::4004 Chemical engineering