Bai, LingrongWang, JingZhou, HuitongGong, HuaTao, JinzhongHickford, Jonathan2019-06-272019-04-022019-042019-03-29Bai et al. (2019). Identification of ovine KRTAP28-1 and its association with wool fibre diameter. Animals, 9(4), 142. doi:10.3390/ani90401422076-2615HX3NK (isidoc)30987059 (pubmed)https://hdl.handle.net/10182/10788Keratin-associated proteins (KAPs) are a diverse group of proteins and form a matrix that cross-links keratin intermediate filaments in hair and wool fibres. From over 100 KAP genes (KRTAPs) identified in mammalian species, KRTAP25-1 is a high sulphur (HS)-KAP gene, which has recently been described in humans. Here, we report the absence of KRTAP25-1 in sheep, and describe a new HS-KRTAP (named KRTAP28-1) in the chromosome region where KRTAP25-1 was expected to be found. Six variants (A−F) of KRTAP28-1 containing eight single nucleotide polymorphisms (SNPs) and a TG repeat polymorphism were detected. One was positioned 30 bp upstream of the transcription start codon and all the others were non-synonymous SNPs, including a nonsense SNP. The TG repeat polymorphism would lead to a reading frame shift at the carboxyl-terminal end. The effect of KRTAP28-1 on wool traits was investigated with 383 Southdown × Merino-cross lambs from seven sire lines. Of the four genotypes with a frequency of over 5%, lambs of genotypes AB and BD produced wool of a smaller MFD than lambs of genotype BC. This shows that KRTAP28-1 is associated with wool fibre diameter, and that variation in this gene might have potential for use as a gene marker for reducing wool fibre diameter.11 pagesElectronicen© The authors. Licensee MDPI, Basel, Switzerland.sheepkeratin-associated protein KAP28-1 gene (KRTAP28-1)polymerase chain reaction (PCR)single-stranded conformational polymorphism (SSCP)variationwool fibre diameterJournal Article10.3390/ani9040142ANZSRC::0702 Animal ProductionANZSRC::070201 Animal BreedingANZSRC::0604 GeneticsANZSRC::060403 Developmental Genetics (incl. Sex Determination)ANZSRC::060411 Population, Ecological and Evolutionary GeneticsANZSRC::060412 Quantitative Genetics (incl. Disease and Trait Mapping Genetics)2076-2615ANZSRC::3003 Animal productionANZSRC::3009 Veterinary sciencesANZSRC::3109 Zoologyhttps://creativecommons.org/licenses/by/4.0/Attribution