Fungal communities in the native New Zealand medicinal plant Pseudowintera colorata (Horopito) are determined by plant organ type and host maturity with key members promoting plant growth

Purushotham Balraj, NJones, ElizabethMonk, JRidgway, H2022-03-092021-12-132021-122021-12-112076-2607XW9PD (isidoc)34946177 (pubmed)https://hdl.handle.net/10182/14679The plant Pseudowintera colorata is well known for its antimicrobial and medicinal properties and is endemic to New Zealand. Using PCR‐Denaturing gradient gel electrophoresis (DGGE), we investigated the factors influencing the composition of endophytic fungal communities in P. colorata from ten distinct sites across New Zealand. Our results showed that plant organs of P. colorata influenced the diversity and richness of endophytic fungi (PERMANOVA, p < 0.05). In addition, plant maturity and its interactions revealed that endophytic fungal communities formed discrete clusters in leaves, stems, and roots of mature and immature P. colorata plants (PERMANOVA; p = 0.002, p = 0.001 and p = 0.039, respectively). For identifying isolates with biocontrol potential, dual culture tests were set up against four different phytopathogenic fungi. Isolates with high activity (zone of inhibition >10 mm) were sequenced and identified as Trichoderma harzianum, Pezicula neosporulosa, Fusarium tricinctum, Metarhizium sp., and Chaetomium sp. Applying selected endo-phytic fungi (n = 7) as soil drenchers significantly increased the growth of P. colorata seedlings and produced more internodes. Seedling shoots treated with Trichoderma sp. PRY2BA21 were 2.2 × longer (8.36 cm) than the untreated controls (3.72 cm). Our results elucidate the main plant factors influencing fungal community composition and demonstrate a role for endophytic fungi in P. colorata growth and further demonstrate that medicinal plants are a rich source of endophytes with potential as biocontrol agents.14 pagesElectronicen© 2021 by the authors. Licensee MDPI, Basel, Switzerland.antifungal activitybiocontrolDGGEendophytesendophytic fungimedicinal plantmicrobial ecologyNew Zealandplant pathogensplant-microbe interactionsFungal communities in the native New Zealand medicinal plant Pseudowintera colorata (Horopito) are determined by plant organ type and host maturity with key members promoting plant growthJournal Article10.3390/microorganisms91225762076-26072022-02-10ANZSRC::410302 Biological controlANZSRC::310703 Microbial ecologyANZSRC::300409 Crop and pasture protection (incl. pests, diseases and weeds)ANZSRC::310805 Plant pathologyANZSRC::310705 MycologyANZSRC::3107 MicrobiologyANZSRC::3207 Medical microbiologyhttps://creativecommons.org/licenses/by/4.0/Attribution