Khoshouei, MRadjainia, MPhillips, AJGerrard, JAMitra, AKPlitzko, JMBaumeister, WDanev, R2018-07-302016-01-282016-01-282015-12-23Khoshouei et al. (2016). Volta phase plate cryo-EM of the small protein complex Prx3. Nature Communications, 7, 10534. doi:10.1038/ncomms10534https://hdl.handle.net/10182/10095Cryo-EM of large, macromolecular assemblies has seen a significant increase in the numbers of high-resolution structures since the arrival of direct electron detectors. However, sub-nanometre resolution cryo-EM structures are rare compared with crystal structure depositions, particularly for relatively small particles (<400 kDa). Here we demonstrate the benefits of Volta phase plates for single-particle analysis by time-efficient cryo-EM structure determination of 257 kDa human peroxiredoxin-3 dodecamers at 4.4 Å resolution. The Volta phase plate improves the applicability of cryo-EM for small molecules and accelerates structure determination.en© The Authors This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/cryoelectron microscopymolecular biophysicsJournal Article10.1038/ncomms10534ANZSRC::06 Biological SciencesANZSRC::0605 MicrobiologyANZSRC::1007 NanotechnologyANZSRC::1001 Agricultural Biotechnology2041-1723https://creativecommons.org/licenses/by/4.0/Attribution