Du, XZhou, HuitongLiu, XLi, YHickford, Jonathan2022-03-292021-11-112021-112021-11-092076-2615XG1DX (isidoc)34827956 (pubmed)https://hdl.handle.net/10182/14786Lipin-1 is known to play a regulatory role in tissues that function in lipid metabolism. In dairy cows, the lipin-1 gene (LPIN1) is highly expressed in the mammary gland, but its function in milk production is less understood. In this study, we used PCR-single strand conformation polymorphism analysis to investigate sequence variation in three regions of bovine LPIN1 in New Zealand Holstein-Friesian × Jersey (HF × J)-cross dairy cows, including part of the 5′ non-coding region, the region containing the LPIN1β-spliced exon, and the sixth coding exon that encodes the putative transcriptional activating domain of the protein. No variation was found in the LPIN1β-spliced exon, but two sequence variants containing one single nucleotide polymorphism (SNP) were identified in the 5′ non-coding region and four sequence variants containing four non-synonymous SNPs were identified in the sixth coding exon. Among the three common variants of the sixth coding exon, variant C was found to be associated with an increase in milk fat percentage (presence 4.96 ± 0.034% vs. absence 4.81 ± 0.050%; p = 0.006) and milk protein percentage (presence 4.09 ± 0.017% vs. absence 3.99 ± 0.025%; p = 0.001), but no associations (p > 0.01) were detected for milk yield. These results suggest that variation in LPIN1 affect the synthesis of fat and proteins in milk and has potential as a gene-marker to improve milk production traits.9 pagesElectronicen© 2021 by the authors. Licensee MDPI, Basel, Switzerland.bovineLPIN1milk fat percentagemilk protein percentagePCR-SSCPJournal Article10.3390/ani111132232076-26152022-02-10ANZSRC::310902 Animal cell and molecular biologyANZSRC::310599 Genetics not elsewhere classifiedANZSRC::300301 Animal growth and developmentANZSRC::3003 Animal productionANZSRC::3009 Veterinary sciencesANZSRC::3109 Zoologyhttps://creativecommons.org/licenses/by/4.0/Attribution