Kerkhof, P-JOn, StephenHouf, K2024-05-072021-11-1920212021-10-011466-502634797211 (pubmed)https://hdl.handle.net/10182/17176A study on the polyphasic taxonomic classification of an Arcobacter strain, R-73987T, isolated from the rectal mucus of a porcine intestinal tract, was performed. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the strain could be assigned to the genus Arcobacter and suggested that strain R-73987T belongs to a novel undescribed species. Comparative analysis of the rpoB gene sequence confirmed the findings. Arcobacter faecis LMG 28519T was identified as its closest neighbour in a multigene analysis based on 107 protein-encoding genes. Further, whole-genome sequence comparisons by means of average nucleotide identity and in silico DNA–DNA hybridization between the genome of strain R-73987T and the genomes of validly named Arcobacter species resulted in values below 95–96 and 70%, respectively. In addition, a phenotypic analysis further corroborated the conclusion that strain R-73987T represents a novel Arcobacter species, for which the name Arcobacter vandammei sp. nov. is proposed. The type strain is R-73987T (=LMG 31429T=CCUG 75005T). This appears to be the first Arcobacter species recovered from porcine intestinal mucus.9 pagesPrinten© 2021 The Authors.ArcobacterArcobacteraceaedigital DNA–DNA hybridizationmucusOrthoANIupigRectumMucusAnimalsSwineSus scrofaArcobacterFatty AcidsDNA, BacterialRNA, Ribosomal, 16SBacterial Typing TechniquesNucleic Acid HybridizationSequence Analysis, DNAPhylogenyBase CompositionJournal Article10.1099/ijsem.0.0051131466-5034ANZSRC::310112 Structural biology (incl. macromolecular modelling)ANZSRC::310114 Systems biologyANZSRC::310902 Animal cell and molecular biologyANZSRC::310506 Gene mappingANZSRC::310505 Gene expression (incl. microarray and other genome-wide approaches)ANZSRC::3104 Evolutionary biologyANZSRC::3107 Microbiology