Determination of bitter acids in South Island grown hops by UPLC : A Dissertation submitted in partial fulfilment of the requirements for the Degree of Master of Science in Food Innovation at Lincoln University

Wu, Bin2021-03-172021-03-172021https://hdl.handle.net/10182/13606In this study, ultra-performance liquid chromatography was used to quantify the content of alpha acids in hops grown in Nelson, Canterbury and Southland. This method used a binary solvent system consisting of (1) 100% methanol (A) and (2) 85% of solvent A and 15% of solvent B. B is 0.17% v/v formic acid and water. Analysis was performed isocratically at a flowrate of 0.2 mL/min. Separation was achieved on the column of Acquity UPLC® BEH C18, 50 x 2.1 mm, with a particle size of 1.7μm, and with a Acquity UPLC® BEH C18 1.7μm, VanGuard™ pre-column 5 x 2.1 mm. The compounds of interest eluted within 4 mins. The results of this experimental study show that the content of alpha acids in hops is not only related to the growth environment but also related to the variety.enUltra Performance Liquid Chromatography (UPLC)Humulus lupulus L.alpha acidsbeta acidsDetermination of bitter acids in South Island grown hops by UPLC : A Dissertation submitted in partial fulfilment of the requirements for the Degree of Master of Science in Food Innovation at Lincoln UniversityDissertationANZSRC::0908 Food SciencesANZSRC::090899 Food Sciences not elsewhere classifiedANZSRC::090801 Food Chemistry and Molecular Gastronomy (excl. Wine)Q112957277