Polymorphism of KRT83 and its association with selected wool traits in Merino-cross lambs
dc.contributor.author | Chai, W | |
dc.contributor.author | Zhou, Huitong | |
dc.contributor.author | Forrest, RHJ | |
dc.contributor.author | Gong, H | |
dc.contributor.author | Hodge, S | |
dc.contributor.author | Hickford, Jonathan | |
dc.date.accessioned | 2017-11-22T20:26:19Z | |
dc.date.available | 2017-08-31 | |
dc.date.issued | 2017-10-01 | |
dc.date.submitted | 2017-08-28 | |
dc.description.abstract | Keratins are structural proteins in the cortex of wool fibres. It is thought that variation in the keratin genes (KRTs) affects wool structure and characteristics. Polymerase chain reaction-single stranded conformational polymorphism (PCR-SSCP) analysis was used to investigate three regions of ovine KRT83. These regions were a portion of the promoter, the exon 2 region (including part of intron 1) and a region encompassing exon 3-4 (including all of exon 3, intron 3, exon 4 and part of intron 4). Initially, in 300 New Zealand Romney, Merino and White Dorper sheep obtained from 26 farms, one, two and four PCR-SSCP banding patterns were observed for these regions respectively. The exon 2 region contained two single nucleotide polymorphisms (SNPs) and the exon 3-4 region contained five SNPs. Investigation of the effect of the variation in the exon 3-4 region on variation in some wool traits were subsequently undertaken in 489 Merino × Southdown-cross sheep from seven sire-lines. The four variants identified in the original 300 sheep (designated A-D) and a new variant (E containing a new SNP) were observed with a frequency of 64.6%, 15.4%, 6.6%, 10.1% and 3.3%, respectively. General linear mixed-effects models (GLMMs) were used to investigate associations between the presence or absence of the variants and the wool traits, with a second set of models testing associations between common genotypes and those traits. The presence of A was associated with a decrease in fibre diameter standard deviation (FDSD) and coefficient of variation of fibre diameter (CVFD). The presence of C was associated with an increase in mean fibre diameter (MFD), mean fibre curvature (MFC) and prickle factor (PF), and a decrease in wool yield (Yield) [clean fleece weight (CFW)/greasy fleece weight (GFW) × 100%]. A trend for association between the presence of C and increased FDSD was also detected. The presence of D was associated with an increase in MFD and PF, and a decrease in Yield. The presence of E was associated with a decrease in CVFD. Genotype AD had a higher GFW and a lower Yield than AA, and AC and AD tended to have increased MFD compared to AA and AB. These results suggest that ovine KRT83 might be a useful candidate gene for improving wool traits. | |
dc.format.extent | pp.6-11 | |
dc.identifier | https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=elements_prod&SrcAuth=WosAPI&KeyUT=WOS:000413282400002&DestLinkType=FullRecord&DestApp=WOS_CPL | |
dc.identifier.citation | Chai, Wenqiong, Zhou, Huitong, Forrest, Rachel H.J., Gong, Hua, Hodge, Simon, Hickford, Jon G.H., Polymorphism of KRT83 and its association with selected wool traits in Merino-cross lambs.Small Ruminant Research http://dx.doi.org/10.1016/j.smallrumres.2017.08.019 | |
dc.identifier.doi | 10.1016/j.smallrumres.2017.08.019 | |
dc.identifier.eissn | 1879-0941 | |
dc.identifier.issn | 0921-4488 | |
dc.identifier.other | FK1ZP (isidoc) | |
dc.identifier.uri | https://hdl.handle.net/10182/8779 | |
dc.language.iso | en | |
dc.publisher | Elsevier | |
dc.relation | The original publication is available from Elsevier - https://doi.org/10.1016/j.smallrumres.2017.08.019 - http://dx.doi.org/10.1016/j.smallrumres.2017.08.019 | |
dc.relation.isPartOf | Small Ruminant Research | |
dc.relation.uri | https://doi.org/10.1016/j.smallrumres.2017.08.019 | |
dc.rights | © 2017 Elsevier B.V. All rights reserved. | |
dc.rights.ccname | Attribution-NonCommercial | |
dc.rights.ccuri | https://creativecommons.org/licenses/by-nc/4.0/ | |
dc.subject | KRT83 | |
dc.subject | variation | |
dc.subject | wool traits | |
dc.subject | sheep | |
dc.subject.anzsrc | ANZSRC::070201 Animal Breeding | |
dc.subject.anzsrc | ANZSRC::070299 Animal Production not elsewhere classified | |
dc.subject.anzsrc2020 | ANZSRC::3003 Animal production | |
dc.title | Polymorphism of KRT83 and its association with selected wool traits in Merino-cross lambs | |
dc.type | Journal Article | |
lu.contributor.unit | LU | |
lu.contributor.unit | LU|Agriculture and Life Sciences | |
lu.contributor.unit | LU|Agriculture and Life Sciences|AGSC | |
lu.contributor.unit | LU|Research Management Office | |
lu.contributor.unit | LU|Research Management Office|OLD QE18 | |
lu.contributor.unit | LU|Centre of Excellence for One Biosecurity Research, Analysis and Synthesis | |
lu.identifier.orcid | 0000-0001-7620-3160 | |
lu.identifier.orcid | 0000-0001-9313-761X | |
pubs.publication-status | Published | |
pubs.publisher-url | http://dx.doi.org/10.1016/j.smallrumres.2017.08.019 | |
pubs.volume | 155 |
Files
Original bundle
1 - 2 of 2
No Thumbnail Available
- Name:
- ChaiZhouForrestGongHodgeHickford-SRR-2017.pdf
- Size:
- 499.19 KB
- Format:
- Adobe Portable Document Format
- Description:
- Accepted Manuscript-1 year embargo
No Thumbnail Available
- Name:
- ChaiZhouForrestGongHodgeHickord-2017.pdf
- Size:
- 693.63 KB
- Format:
- Adobe Portable Document Format
- Description:
- Journal Article (publisher version - embargoed - use DOI)
Licence bundle
1 - 1 of 1
No Thumbnail Available
- Name:
- Symplectic Collection licence doc.docx
- Size:
- 11.3 KB
- Format:
- Microsoft Word 2007
- Description: