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    Transmission of ovine footrot : a dissertation submitted in partial fulfilment of the requirements for the degree of Bachelor of Science with Honours at Lincoln University

    Pearce, Annette L.
    Abstract
    Ovine footrot is a contagious bacterial disease caused by Dichelobacter nodosus. It is endemic to New Zealand and other sheep producing regions of the world and results in significant economic losses. Transmission of this bacterium is not well understood. It is thought to spread from host to host via contaminated soil. It has been suggested that other vectors may move the contaminated soil about on their feet, promoting transmission. To address the possibility of other vectors spreading the disease, primers specific to the fimA gene of D. nodosus were used to detect its presence in DNA derived from soil collected off paddocks containing footrot infected sheep, and soil and lesion material collected from the hooves of infected animals. D. nodosus was detected in lesion material, but not from DNA derived from the soil. The sensitivity of the fimA PCR was assessed and further extraction procedures were attempted to improve the purity of the DNA. It was concluded that the PCR was highly specific to D. nodosus, but the sensitivity of the reaction may have been a limitation to the analysis of the soil collected.... [Show full abstract]
    Keywords
    Dichelobacter nodosus; fimA; transmission; vector; ovine footrot; Polymerase Chain Reaction
    Fields of Research
    070205 Animal Protection (Pests and Pathogens); 060501 Bacteriology
    Date
    1999
    Type
    Dissertation
    Access Rights
    Digital thesis can be viewed by current staff and students of Lincoln University only. Print copy available for reading in Lincoln University Library.
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    • Theses and Dissertations with Restricted Access [2179]
    • Department of Agricultural Sciences [1398]
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