Simultaneous quantification of aflatoxin B₁, T-2 toxin, ochratoxin A and deoxynivalenol in dried seafood products by LC-MS/MS
dc.contributor.author | Deng, Yijia | |
dc.contributor.author | Wang, Yaling | |
dc.contributor.author | Deng, Qi | |
dc.contributor.author | Sun, Lijun | |
dc.contributor.author | Wang, Rundong | |
dc.contributor.author | Wang, Xiaobo | |
dc.contributor.author | Liao, Jianmeng | |
dc.contributor.author | Gooneratne, R | |
dc.coverage.spatial | Switzerland | |
dc.date.accessioned | 2020-12-14T02:44:43Z | |
dc.date.available | 2020-07-30 | |
dc.date.issued | 2020-08 | |
dc.date.submitted | 2020-07-29 | |
dc.description.abstract | Mycotoxins are secondary metabolites produced by fungi. These contaminate dried seafoods during processing and storage and represent a potential health hazard for consumers. A sensitive, selective and accurate liquid chromatography/tandem mass spectrometry (LC-MS/MS) method was established for simultaneous quantification of four common mycotoxins (aflatoxin B₁ (AFB₁), T-2 toxin (T-2), ochratoxin A (OTA) and deoxynivalenol (DON)) in dried shrimp, dried fish and dried mussel products. Mycotoxins were extracted from dried seafood samples by acetonitrile/water (85/15, v/v), subjected to ultrasound for 60 min at 20°C and cleaned up by defatting with n-hexane. The sample matrix affected the linearity of detection (R² ≥ 0.9974). The limit of detection (LOD) and limit of quantification (LOQ) in dried seafood products varied from 0.1 to 2.0 μg.kg⁻¹and 0.3 to 5.0 μg.kg⁻¹, respectively. The method was validated by spiking samples with specific mycotoxin levels, and the recoveries, intra-relative standard deviation (RSDs) and inter-RSDs ranged between 72.2-98.4%, 2.8-10.6%, and 5.5-15.4%, respectively. This method was used to analyze 40 dried seafood products purchased from the Zhanjiang seafood market. Results of this product sampling showed that while no DON was detected, AFB₁, T-2 and OTA were detected in 30.8%, 17.5% and 33.3% of the samples, respectively. AFB₁, T-2 and OTA concentrations varied at 0.58-0.89, 0.55-1.34 and 0.36-1.51 μg.kg⁻¹, respectively. Relatively high frequency of contamination and the presence of AFB₁, OTA and T-2 residues indicate the need to monitor mycotoxins in dried seafood products. | |
dc.format.extent | 13 pages | |
dc.format.medium | Electronic | |
dc.identifier | toxins12080488 | |
dc.identifier | https://www.ncbi.nlm.nih.gov/pubmed/32751656 | |
dc.identifier.doi | 10.3390/toxins12080488 | |
dc.identifier.eissn | 2072-6651 | |
dc.identifier.issn | 2072-6651 | |
dc.identifier.other | 32751656 (pubmed) | |
dc.identifier.uri | https://hdl.handle.net/10182/13157 | |
dc.language.iso | en | |
dc.publisher | MDPI | |
dc.relation | The original publication is available from MDPI - https://doi.org/10.3390/toxins12080488 - http://dx.doi.org/10.3390/toxins12080488 | |
dc.relation.isPartOf | Toxins | |
dc.relation.uri | https://doi.org/10.3390/toxins12080488 | |
dc.rights | © 2020 by the authors. Licensee MDPI, Basel, Switzerland. | |
dc.rights.ccname | Attribution | |
dc.rights.ccuri | https://creativecommons.org/licenses/by/4.0/ | |
dc.subject | mycotoxins | |
dc.subject | dried seafood | |
dc.subject | liquid chromatography-tandem mass spectrometry | |
dc.subject | method validation | |
dc.subject | ultrasound-assisted extraction | |
dc.subject.anzsrc | ANZSRC::0605 Microbiology | |
dc.subject.anzsrc | ANZSRC::0908 Food Sciences | |
dc.subject.anzsrc | ANZSRC::111506 Toxicology (incl. Clinical Toxicology) | |
dc.subject.anzsrc | ANZSRC::030101 Analytical Spectrometry | |
dc.subject.mesh | Animals | |
dc.subject.mesh | Fishes | |
dc.subject.mesh | Penaeidae | |
dc.subject.mesh | Trichothecenes | |
dc.subject.mesh | T-2 Toxin | |
dc.subject.mesh | Ochratoxins | |
dc.subject.mesh | Aflatoxin B1 | |
dc.subject.mesh | Chromatography, High Pressure Liquid | |
dc.subject.mesh | Food Contamination | |
dc.subject.mesh | Seafood | |
dc.subject.mesh | Mytilus edulis | |
dc.subject.mesh | Tandem Mass Spectrometry | |
dc.subject.mesh | Aflatoxin B1 | |
dc.subject.mesh | Animals | |
dc.subject.mesh | Chromatography, High Pressure Liquid | |
dc.subject.mesh | Fishes | |
dc.subject.mesh | Food Contamination | |
dc.subject.mesh | Mytilus edulis | |
dc.subject.mesh | Ochratoxins | |
dc.subject.mesh | Penaeidae | |
dc.subject.mesh | Seafood | |
dc.subject.mesh | T-2 Toxin | |
dc.subject.mesh | Tandem Mass Spectrometry | |
dc.subject.mesh | Trichothecenes | |
dc.title | Simultaneous quantification of aflatoxin B₁, T-2 toxin, ochratoxin A and deoxynivalenol in dried seafood products by LC-MS/MS | |
dc.type | Journal Article | |
lu.contributor.unit | Lincoln University | |
lu.contributor.unit | Faculty of Agriculture and Life Sciences | |
lu.contributor.unit | Department of Wine, Food and Molecular Biosciences | |
lu.identifier.orcid | 0000-0002-5406-2894 | |
pubs.article-number | 488 | |
pubs.issue | 8 | |
pubs.publication-status | Published | |
pubs.publisher-url | http://dx.doi.org/10.3390/toxins12080488 | |
pubs.volume | 12 |
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