The effects of dietary nitrogen to water-soluble carbohydrate ratio on isotopic fractionation and partitioning of nitrogen in non-lactating sheep
dc.contributor.author | Cheng, L | |
dc.contributor.author | Nicol, AM | |
dc.contributor.author | Dewhurst, RJ | |
dc.contributor.author | Edwards, GR | |
dc.coverage.spatial | England | |
dc.date.accessioned | 2017-03-23T03:27:28Z | |
dc.date.available | 2013-03-18 | |
dc.date.issued | 2013-08 | |
dc.date.submitted | 2013-02-05 | |
dc.description.abstract | The main objective of this study was to investigate the relationship between partitioning and isotopic fractionation of nitrogen (N) in sheep consuming diets with varying ratios of N to water-soluble carbohydrate (WSC). Six non-lactating sheep were offered a constant dry matter (DM) allowance with one of three ratios of dietary N/WSC, achieved by adding sucrose and urea to lucerne pellets. A replicated 3 dietary treatments (Low, Medium and High N/WSC) × 3 (collection periods) and a Latin square design was used, with two sheep assigned to each treatment in each period. Feed, faeces, urine, plasma, wool, muscle and liver samples were collected and analysed for ¹⁵N concentration. Nitrogen intake and outputs in faeces and urine were measured for each sheep using 6-day total collections. Blood urea N (BUN) and urinary excretion of purine derivative were also measured. Treatment effects were tested using general ANOVA; the relationships between measured variables were analysed by linear regression. BUN and N intake increased by 46% and 35%, respectively, when N/WSC increased 2.5-fold. However, no indication of change in microbial protein synthesis was detected. Results indicated effects of dietary treatments on urinary N/faecal N, faecal N/N intake and retained N/N intake. In addition, the linear relationships between plasma δ¹⁵N and urinary N/N intake and muscle δ¹⁵N and retained N/N intake based on individual measurements showed the potential of using N isotopic fractionation as an easy-to-use indicator of N partitioning when N supply exceeds that required to match energy supply in the diet. | |
dc.format.extent | pp.1274-1279 | |
dc.format.medium | Print-Electronic | |
dc.identifier | S1751731113000311 | |
dc.identifier | https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=elements_prod&SrcAuth=WosAPI&KeyUT=WOS:000321220800007&DestLinkType=FullRecord&DestApp=WOS_CPL | |
dc.identifier.citation | Cheng, L., Nicol, A.M., Dewhurst, R.J., & Edwards, G.R. (2013). The effects of dietary nitrogen to water-soluble carbohydrate ratio on isotopic fractionation and partitioning of nitrogen in non-lactating sheep. Animal, 7(8), 1274-1279. doi:10.1017/S1751731113000311 | |
dc.identifier.doi | 10.1017/S1751731113000311 | |
dc.identifier.eissn | 1751-732X | |
dc.identifier.issn | 1751-7311 | |
dc.identifier.other | 23497973 (pubmed) | |
dc.identifier.uri | https://hdl.handle.net/10182/7934 | |
dc.language.iso | en | |
dc.publisher | Cambridge University Press (CUP) on behalf of the Animal Consortium | |
dc.relation | The original publication is available from Cambridge University Press (CUP) on behalf of the Animal Consortium - https://doi.org/10.1017/S1751731113000311 - http://dx.doi.org/10.1017/s1751731113000311 | |
dc.relation.isPartOf | Animal | |
dc.relation.uri | https://doi.org/10.1017/S1751731113000311 | |
dc.rights | © The Animal Consortium 2013 | |
dc.rights.ccname | Attribution-NonCommercial-NoDerivatives | |
dc.rights.ccuri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.subject | stable isotope | |
dc.subject | water-soluble carbohydrates | |
dc.subject | urinary nitrogen | |
dc.subject | purine derivatives | |
dc.subject.anzsrc | ANZSRC::0702 Animal Production | |
dc.subject.anzsrc | ANZSRC::070204 Animal Nutrition | |
dc.subject.anzsrc2020 | ANZSRC::30 Agricultural, veterinary and food sciences | |
dc.subject.anzsrc2020 | ANZSRC::31 Biological sciences | |
dc.subject.mesh | Feces | |
dc.subject.mesh | Animals | |
dc.subject.mesh | Sheep, Domestic | |
dc.subject.mesh | Nitrogen | |
dc.subject.mesh | Nitrogen Isotopes | |
dc.subject.mesh | Urea | |
dc.subject.mesh | Dietary Carbohydrates | |
dc.subject.mesh | Sucrose | |
dc.subject.mesh | Blood Urea Nitrogen | |
dc.subject.mesh | Chemical Fractionation | |
dc.subject.mesh | Analysis of Variance | |
dc.subject.mesh | Animal Feed | |
dc.subject.mesh | Male | |
dc.subject.mesh | Mass Spectrometry | |
dc.title | The effects of dietary nitrogen to water-soluble carbohydrate ratio on isotopic fractionation and partitioning of nitrogen in non-lactating sheep | |
dc.type | Journal Article | |
lu.contributor.unit | LU | |
lu.contributor.unit | LU|Agriculture and Life Sciences | |
lu.contributor.unit | LU|Agriculture and Life Sciences|AGSC | |
lu.contributor.unit | LU|Research Management Office | |
lu.contributor.unit | LU|Vice Chancellor's Office | |
lu.contributor.unit | LU|Research Management Office|OLD QE18 | |
lu.identifier.orcid | 0000-0003-4165-007X | |
pubs.issue | 8 | |
pubs.publication-status | Published | |
pubs.publisher-url | http://dx.doi.org/10.1017/s1751731113000311 | |
pubs.volume | 7 |
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