The effects of dietary nitrogen to water-soluble carbohydrate ratio on isotopic fractionation and partitioning of nitrogen in non-lactating sheep

dc.contributor.authorCheng, L
dc.contributor.authorNicol, AM
dc.contributor.authorDewhurst, RJ
dc.contributor.authorEdwards, GR
dc.coverage.spatialEngland
dc.date.accessioned2017-03-23T03:27:28Z
dc.date.available2013-03-18
dc.date.issued2013-08
dc.date.submitted2013-02-05
dc.description.abstractThe main objective of this study was to investigate the relationship between partitioning and isotopic fractionation of nitrogen (N) in sheep consuming diets with varying ratios of N to water-soluble carbohydrate (WSC). Six non-lactating sheep were offered a constant dry matter (DM) allowance with one of three ratios of dietary N/WSC, achieved by adding sucrose and urea to lucerne pellets. A replicated 3 dietary treatments (Low, Medium and High N/WSC) × 3 (collection periods) and a Latin square design was used, with two sheep assigned to each treatment in each period. Feed, faeces, urine, plasma, wool, muscle and liver samples were collected and analysed for ¹⁵N concentration. Nitrogen intake and outputs in faeces and urine were measured for each sheep using 6-day total collections. Blood urea N (BUN) and urinary excretion of purine derivative were also measured. Treatment effects were tested using general ANOVA; the relationships between measured variables were analysed by linear regression. BUN and N intake increased by 46% and 35%, respectively, when N/WSC increased 2.5-fold. However, no indication of change in microbial protein synthesis was detected. Results indicated effects of dietary treatments on urinary N/faecal N, faecal N/N intake and retained N/N intake. In addition, the linear relationships between plasma δ¹⁵N and urinary N/N intake and muscle δ¹⁵N and retained N/N intake based on individual measurements showed the potential of using N isotopic fractionation as an easy-to-use indicator of N partitioning when N supply exceeds that required to match energy supply in the diet.
dc.format.extentpp.1274-1279
dc.format.mediumPrint-Electronic
dc.identifierS1751731113000311
dc.identifierhttps://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=elements_prod&SrcAuth=WosAPI&KeyUT=WOS:000321220800007&DestLinkType=FullRecord&DestApp=WOS
dc.identifier.citationCheng, L., Nicol, A.M., Dewhurst, R.J., & Edwards, G.R. (2013). The effects of dietary nitrogen to water-soluble carbohydrate ratio on isotopic fractionation and partitioning of nitrogen in non-lactating sheep. Animal, 7(8), 1274-1279. doi:10.1017/S1751731113000311
dc.identifier.doi10.1017/S1751731113000311
dc.identifier.eissn1751-732X
dc.identifier.issn1751-7311
dc.identifier.other23497973 (pubmed)
dc.identifier.urihttps://hdl.handle.net/10182/7934
dc.language.isoen
dc.publisherCambridge University Press (CUP) on behalf of the Animal Consortium
dc.relationThe original publication is available from Cambridge University Press (CUP) on behalf of the Animal Consortium - https://doi.org/10.1017/S1751731113000311 - http://dx.doi.org/10.1017/s1751731113000311
dc.relation.isPartOfAnimal
dc.relation.urihttps://doi.org/10.1017/S1751731113000311
dc.rights© The Animal Consortium 2013
dc.rights.ccnameAttribution-NonCommercial-NoDerivatives
dc.rights.ccurihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectstable isotope
dc.subjectwater-soluble carbohydrates
dc.subjecturinary nitrogen
dc.subjectpurine derivatives
dc.subject.anzsrcANZSRC::0702 Animal Production
dc.subject.anzsrcANZSRC::070204 Animal Nutrition
dc.subject.anzsrc2020ANZSRC::30 Agricultural, veterinary and food sciences
dc.subject.anzsrc2020ANZSRC::31 Biological sciences
dc.subject.meshFeces
dc.subject.meshAnimals
dc.subject.meshSheep, Domestic
dc.subject.meshNitrogen
dc.subject.meshNitrogen Isotopes
dc.subject.meshUrea
dc.subject.meshDietary Carbohydrates
dc.subject.meshSucrose
dc.subject.meshBlood Urea Nitrogen
dc.subject.meshChemical Fractionation
dc.subject.meshAnalysis of Variance
dc.subject.meshAnimal Feed
dc.subject.meshMale
dc.subject.meshMass Spectrometry
dc.titleThe effects of dietary nitrogen to water-soluble carbohydrate ratio on isotopic fractionation and partitioning of nitrogen in non-lactating sheep
dc.typeJournal Article
lu.contributor.unitLU
lu.contributor.unitLU|Agriculture and Life Sciences
lu.contributor.unitLU|Agriculture and Life Sciences|AGSC
lu.contributor.unitLU|Research Management Office
lu.contributor.unitLU|Vice Chancellor's Office
lu.contributor.unitLU|Research Management Office|OLD QE18
lu.identifier.orcid0000-0003-4165-007X
pubs.issue8
pubs.publication-statusPublished
pubs.publisher-urlhttp://dx.doi.org/10.1017/s1751731113000311
pubs.volume7
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