Identification of the caprine keratin-associated protein 20-2 (KAP20-2) gene and its effect on cashmere traits
dc.contributor.author | Wang, J | |
dc.contributor.author | Che, L | |
dc.contributor.author | Hickford, Jonathan | |
dc.contributor.author | Zhou, Huitong | |
dc.contributor.author | Hao, Z | |
dc.contributor.author | Luo, Y | |
dc.contributor.author | Hu, J | |
dc.contributor.author | Liu, X | |
dc.contributor.author | Li, S | |
dc.coverage.spatial | Switzerland | |
dc.date.accessioned | 2018-03-26T00:28:52Z | |
dc.date.available | 2017-11-17 | |
dc.date.issued | 2017-11 | |
dc.date.submitted | 2017-11-13 | |
dc.description.abstract | The gene encoding the high glycine/tyrosine keratin-associated protein 20-2 (KAP20-2) gene has been described in humans, but has not been identified in any livestock species. A search for similar sequences in the caprine genome using the human KAP20-2 gene (KRTAP20-2) revealed a homologous sequence on chromosome 1. Three different banding patterns representing distinct sequences (A–C) in Longdong cashmere goats were identified using polymerase chain reaction-single stranded conformational polymorphism (PCR-SSCP) analysis. These sequences shared high sequence similarity with the human and mouse KRTAP20-2 sequences, suggesting that A–C are caprine variants of the human and mouse genes. Four single nucleotide polymorphisms (SNPs) were identified, and three of them were non-synonymous. KRTAP20-2 was found to be expressed in secondary hair follicles, but not in heart, liver, lung, kidney, spleen, or longissimus dorsi muscle. The presence of A was associated with increased cashmere fibre weight, while the presence of B was associated with a decrease in cashmere fibre weight and curly fibre length. Goats with genotype AA had a higher cashmere fibre weight and a higher curly fibre length than those with genotypes AB or BB. These results indicate that caprine KRTAP20-2 variation may have value as a genetic marker for improving cashmere fibre weight. | |
dc.format.extent | 12 pages | |
dc.format.medium | Electronic | |
dc.identifier | genes8110328 | |
dc.identifier | https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=elements_prod&SrcAuth=WosAPI&KeyUT=WOS:000416800600038&DestLinkType=FullRecord&DestApp=WOS_CPL | |
dc.identifier.citation | Wang et al. (2017). Identification of caprine keratin-associated protein 20-2 (KAP20-2) gene and its effect on cashmere traits. Genes, 8(11), 328. doi:10.3390/genes8110328 | |
dc.identifier.doi | 10.3390/genes8110328 | |
dc.identifier.eissn | 2073-4425 | |
dc.identifier.issn | 2073-4425 | |
dc.identifier.other | FO4GS (isidoc) | |
dc.identifier.other | 29149036 (pubmed) | |
dc.identifier.uri | https://hdl.handle.net/10182/9190 | |
dc.language | eng | |
dc.language.iso | eng | |
dc.publisher | MDPI | |
dc.relation | The original publication is available from MDPI - https://doi.org/10.3390/genes8110328 - http://dx.doi.org/10.3390/genes8110328 | |
dc.relation.isPartOf | Genes | |
dc.relation.uri | https://doi.org/10.3390/genes8110328 | |
dc.rights | © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | |
dc.rights.ccname | Attribution | |
dc.rights.ccuri | https://creativecommons.org/licenses/by/4.0/ | |
dc.subject | cashmere fibre weight | |
dc.subject | keratin-associated protein 20-2 (KAP20-2) gene | |
dc.subject | length of the curly fibre | |
dc.subject | Longdong cashmere goat | |
dc.subject | tissue expression | |
dc.subject | variation | |
dc.subject.anzsrc | ANZSRC::060412 Quantitative Genetics (incl. Disease and Trait Mapping Genetics) | |
dc.subject.anzsrc | ANZSRC::0604 Genetics | |
dc.subject.anzsrc | ANZSRC::0702 Animal Production | |
dc.subject.anzsrc2020 | ANZSRC::3105 Genetics | |
dc.title | Identification of the caprine keratin-associated protein 20-2 (KAP20-2) gene and its effect on cashmere traits | |
dc.type | Journal Article | |
lu.contributor.unit | LU | |
lu.contributor.unit | LU|Agriculture and Life Sciences | |
lu.contributor.unit | LU|Agriculture and Life Sciences|AGSC | |
lu.contributor.unit | LU|Research Management Office | |
lu.contributor.unit | LU|Research Management Office|OLD QE18 | |
lu.contributor.unit | LU|Centre of Excellence for One Biosecurity Research, Analysis and Synthesis | |
lu.identifier.orcid | 0000-0001-9313-761X | |
lu.identifier.orcid | 0000-0001-7620-3160 | |
pubs.issue | 11 | |
pubs.notes | Date of acceptance: 13 Nov 2017 Article number 328 | |
pubs.publication-status | Published online | |
pubs.publisher-url | http://dx.doi.org/10.3390/genes8110328 | |
pubs.volume | 8 |
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