The mean staple length of wool fibre is associated with variation in the ovine keratin-associated protein 21-2 gene
dc.contributor.author | Li, Shaobin, | |
dc.contributor.author | Zhou, Huitong | |
dc.contributor.author | Gong, H | |
dc.contributor.author | Zhao, F | |
dc.contributor.author | Wang, Jiqing, | |
dc.contributor.author | Liu, Xiu, | |
dc.contributor.author | Hu, Jiang, | |
dc.contributor.author | Luo, Yuzhu, | |
dc.contributor.author | Hickford, Jonathan | |
dc.coverage.spatial | Switzerland | |
dc.date.accessioned | 2020-04-01T01:52:28Z | |
dc.date.available | 2020-01-30 | |
dc.date.issued | 2020-02 | |
dc.date.submitted | 2020-01-24 | |
dc.description.abstract | Wool and hair fibres consist of a variety of proteins, including the keratin-associated proteins (KAPs). In this study, a putative ovine homologue of the human KAP21-2 gene (KRTAP21-2) was identified. It was located on chromosome 1 as a 201-bp open reading frame (ORF) in the ovine genome assembly from a Texel sheep (v.4 NC_019458.2: nt122932727 to 122932927). A polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis of this ORF, and subsequent DNA sequencing, identified five sequences (named A-E). The putative amino acid sequences that would be produced, shared some identity with each other and with other KAPs, but they were most similar to ovine KAP21-1, and phylogenetically related to human KAP21-2. The location of the ovine KRTAP21-2 sequence was consistent with the location of human KRTAP21-2, and this suggests they represent different variant forms of ovine KRTAP21-2. Variation in this gene was investigated in 389 Merino (sire) × Southdown-cross (ewe) lambs. These were derived from four independent sire-lines. The sequence variation was found to be associated with variation in five wool traits: including mean staple length (MSL), mean fibre diameter (MFD), fibre diameter standard deviation (FDSD), prickle factor (PF), and greasy fleece weight (GFW). The most persistent effect of KRTAP21-2 variation was with variation in MSL; with the MSL of sheep of genotype AC being 12.5% greater than those of genotype CE. A similar effect was observed from individual variant absence/presence models. This suggests that KRTAP21-2 should be further investigated as a possible gene-marker for improving MSL. | |
dc.format.extent | 10 pages | |
dc.format.medium | Electronic | |
dc.identifier | genes11020148 | |
dc.identifier | https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=elements_prod&SrcAuth=WosAPI&KeyUT=WOS:000519271500099&DestLinkType=FullRecord&DestApp=WOS_CPL | |
dc.identifier.doi | 10.3390/genes11020148 | |
dc.identifier.eissn | 2073-4425 | |
dc.identifier.issn | 2073-4425 | |
dc.identifier.other | 32019077 (pubmed) | |
dc.identifier.uri | https://hdl.handle.net/10182/11677 | |
dc.language.iso | en | |
dc.publisher | MDPI | |
dc.relation | The original publication is available from MDPI - https://doi.org/10.3390/genes11020148 - http://dx.doi.org/10.3390/genes11020148 | |
dc.relation.isPartOf | Genes | |
dc.relation.uri | https://doi.org/10.3390/genes11020148 | |
dc.rights | © 2020 by the authors. | |
dc.rights.ccname | Attribution | |
dc.rights.ccuri | https://creativecommons.org/licenses/by/4.0/ | |
dc.subject | KAP21-2 gene (KRTAP21-2) | |
dc.subject | mean staple length | |
dc.subject | wool traits | |
dc.subject | sheep | |
dc.subject.anzsrc | ANZSRC::0604 Genetics | |
dc.subject.anzsrc | ANZSRC::0702 Animal Production | |
dc.subject.anzsrc2020 | ANZSRC::3105 Genetics | |
dc.subject.mesh | Chromosomes, Mammalian | |
dc.subject.mesh | Animals | |
dc.subject.mesh | Sheep | |
dc.subject.mesh | Chromosome Mapping | |
dc.subject.mesh | Sequence Analysis, DNA | |
dc.subject.mesh | Phylogeny | |
dc.subject.mesh | Sequence Homology, Nucleic Acid | |
dc.subject.mesh | Keratins | |
dc.subject.mesh | Wool Fiber | |
dc.title | The mean staple length of wool fibre is associated with variation in the ovine keratin-associated protein 21-2 gene | |
dc.type | Journal Article | |
lu.contributor.unit | LU | |
lu.contributor.unit | LU|Agriculture and Life Sciences | |
lu.contributor.unit | LU|Agriculture and Life Sciences|AGSC | |
lu.contributor.unit | LU|Research Management Office | |
lu.contributor.unit | LU|Research Management Office|OLD QE18 | |
lu.contributor.unit | LU|Centre of Excellence for One Biosecurity Research, Analysis and Synthesis | |
lu.identifier.orcid | 0000-0001-7620-3160 | |
lu.identifier.orcid | 0000-0001-9313-761X | |
pubs.issue | 2 | |
pubs.notes | Article number 148 | |
pubs.publication-status | Published | |
pubs.publisher-url | http://dx.doi.org/10.3390/genes11020148 | |
pubs.volume | 11 |
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