Anthropogenic N input increases global warming potential by awakening the “sleeping” ancient C in deep critical zones

Abstract

Even a small net increase in soil organic carbon (SOC) mineralization will cause a substantial increase in the atmospheric CO₂ concentration. It is widely recognized that the SOC mineralization within deep critical zones (2 to 12 m depth) is slower and much less influenced by anthropogenic disturbance when compared to that of surface soil. Here, we showed that 20 years of nitrogen (N) fertilization enriched a deep critical zone with nitrate, almost doubling the SOC mineralization rate. This result was supported by corresponding increases in the expressions of functional genes typical of recalcitrant SOC degradation and enzyme activities. The CO₂ released and the SOC had a similar ¹⁴C age (6000 to 10,000 years before the present). Our results indicate that N fertilization of crops may enhance CO₂ emissions from deep critical zones to the atmosphere through a previously disregarded mechanism. This provides another reason for markedly improving N management in fertilized agricultural soils.