Co-transformation of the sclerotial mycoparasite Coniothyrium minitans with hygromycin B resistance and [beta]-glucuronidase markers
dc.contributor.author | Jones, Elizabeth | |
dc.contributor.author | Carpenter, MA | |
dc.contributor.author | Fong, D | |
dc.contributor.author | Goldstein, AL | |
dc.contributor.author | Thrush, A | |
dc.contributor.author | Crowhurst, RN | |
dc.contributor.author | Stewart, A | |
dc.date.accessioned | 2008-03-26T00:34:16Z | |
dc.date.issued | 1999-08 | |
dc.description.abstract | Coniothyrium minitans was successfully co-transformed with the uidA (β-glucuronidase) and the hygromycin-resistance (hph) genes. Both were under the control of the glyceraldehyde-3-phosphate promoter from Aspergillus nidulans. Hygromycin resistance was used as a selectable marker for transformation. In successive transformation experiments, transformation frequencies of up to 1000 transformants µg⁻¹ of plasmid DNA were obtained for isolate A69. Of the ten monospore hygromycin-resistant cultures tested, nine also expressed the uidA gene. Expression of hph and uidA was stable in all transformants after several months of successive subculturing on non-selective medium, and after passage through a sclerotium of Sclerotinia sclerotiorum. Southern hybridization analyses showed all transformants carried multiple copies of each marker gene. When grown on PDA, the culture morphology of three of the transformants of (T×2, T×3 and T×4) was similar to the wild type. Four of the five transformants (T×3, T×4, T×21 and T×24) grew as well as the wild type on different media, and responded to changes in water potential in a similar manner to the wild type. All five transformants were equally parasitic on sclerotia of S. sclerotiorum compared with the wild type. Transformants T×3 and T×4 were the most similar to the wild type in biological characteristics and will be used in future studies. The results indicate that hph- and uidA-transformed strains of C. minitans will be useful for ecological studies on its survival and dissemination. | |
dc.format.extent | pp.929-937 | |
dc.format.medium | Undetermined | |
dc.identifier | https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=elements_prod&SrcAuth=WosAPI&KeyUT=WOS:000081884800001&DestLinkType=FullRecord&DestApp=WOS_CPL | |
dc.identifier.citation | Jones, E., Carpenter, M., Fong, D., Goldstein, A., Thrush, A., Crowhurst, R., & Stewart, A. (1999). Co-transformation of the sclerotial mycoparasite Coniothyrium minitans with hygromycin B resistance and β-glucuronidase markers. Mycological Research, 103(8), 929-937. | |
dc.identifier.doi | 10.1017/S0953756298008168 | |
dc.identifier.eissn | 1469-8102 | |
dc.identifier.issn | 0953-7562 | |
dc.identifier.other | 224EQ (isidoc) | |
dc.identifier.uri | https://hdl.handle.net/10182/375 | |
dc.language.iso | en | |
dc.publisher | Cambridge University Press | |
dc.relation | The original publication is available from Cambridge University Press - https://doi.org/10.1017/S0953756298008168 - http://hdl.handle.net/10182/375 | |
dc.relation.isPartOf | Mycological Research | |
dc.relation.uri | https://doi.org/10.1017/S0953756298008168 | |
dc.rights | Copyright © The British Mycological Society 1999 | |
dc.subject | Coniothyrium minitans | |
dc.subject | mycoparasite | |
dc.subject | marker genes | |
dc.subject.anzsrc2020 | ANZSRC::3107 Microbiology | |
dc.subject.anzsrc2020 | ANZSRC::3108 Plant biology | |
dc.subject.marsden | Marsden::270305 Mycology | |
dc.title | Co-transformation of the sclerotial mycoparasite Coniothyrium minitans with hygromycin B resistance and [beta]-glucuronidase markers | |
dc.type | Journal Article | |
lu.contributor.unit | LU | |
lu.contributor.unit | LU|Agriculture and Life Sciences | |
lu.contributor.unit | LU|Agriculture and Life Sciences|ECOL | |
lu.contributor.unit | LU|OLD BPRC | |
lu.contributor.unit | LU|Research Management Office | |
lu.contributor.unit | LU|Research Management Office|OLD QE18 | |
lu.contributor.unit | LU|Research Management Office|OLD PE20 | |
lu.contributor.unit | LU|Centre of Excellence for One Biosecurity Research, Analysis and Synthesis | |
lu.identifier.orcid | 0000-0002-1879-4537 | |
pubs.issue | 8 | |
pubs.publication-status | Published | |
pubs.publisher-url | http://hdl.handle.net/10182/375 | |
pubs.volume | 103 |
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