Oxalate content in commercially produced cocoa and dark chocolate

Abstract

Cocoa and dark chocolate have been promoted as health foods due to the high levels of antioxidants found in cocoa beans (Theobroma cacao L.) and their products but they also contain moderate to high levels of oxalates which can cause some health concerns. Fifteen samples of commercially available cocoa powder were collected from four different countries and the total and soluble oxalate content was analysed by HPLC chromatography. The total oxalate contents ranged from 650 to 783 mg/100 g dry matter (DM), mean 729 ± 8.4 mg/100 g DM, while the soluble oxalate contents ranged from 360 to 567. mg/100 g DM, mean 469 ± 15 mg/100 g DM. The total oxalate contents of 34 samples of dark chocolate collected from 13 different countries ranged from 155 to 485 mg/100 g DM, mean 254 ± 12 mg/100 g DM while the soluble oxalate contents ranged from 157 to 351 mg/100 g DM, mean 216 ± 10 mg/100 g DM. Oxalate bioavailability was determined by feeding 68.0 ± 0.7 g of dark chocolate containing 232.0 ± 2.3 mg total oxalate as a test meal to 14 volunteers. The mean availability of total oxalate in the chocolate measured from the increase in urinary oxalate output over the following 6 h was 1.82 ± 0.27%. © 2011 Elsevier Inc.