Research@Lincoln
    • Login
     
    View Item 
    •   Research@Lincoln Home
    • Theses and Dissertations
    • Dissertations
    • View Item
    •   Research@Lincoln Home
    • Theses and Dissertations
    • Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Southwestern blotting to establish the composition of UGPB, a putative transcription factor that binds to the rabbit uteroglobin promotor

    Rayner, Julian
    Abstract
    Southwestern blotting identified two major proteins in heparin-agarose purified endometrial nuclear extract that bind to the -194/+9 region of the rabbit uteroglobin promoter, p105 and p70. These proteins were presented as candidate components of the uteroglobin promotor binding complex (UGPB), a protein complex proposed to regulate expression of the endometrial uteroglobin gene. Both p105 and p70 displayed similar binding characteristics as UGPB and p105 was established to co-purify with UGPB during affinity purification. Inclusion of competing DNA in Southwestern blotting suggested that p105 is a relatively non-specific DNA binding protein, whereas p70 is more specific. Both p105 and p70 were shown to bind to the UGPB DNase I footprint region of the uteroglobin promoter (-170/-85) and it was suggested that p105 may bind distally to the uteroglobin transcription start site, while p70 binds proximally. p105 and p70 were identified in unstimulated endometrial extracts as well as endometrial extracts from progesterone and oestrogen stimulated rabbits. pl05 may also be present in unstimulated and oestrogen-dominated lung extracts and in oestrogen-dominated ovary extracts. Several minor proteins were identified in heparin-agarose purified endometrium that may also play a role in the UGPB complex.... [Show full abstract]
    Keywords
    uteroglobin; promoter; transcription factor; Southwestern blotting; gel shift assay; uteroglobin promoter binding factor (UGPB); p105; p70; competition; binding site
    Fields of Research
    0601 Biochemistry and Cell Biology; 0604 Genetics
    Date
    1992
    Type
    Dissertation
    Collections
    • Dissertations [423]
    • Department of Wine, Food and Molecular Biosciences [597]
    Share this

    on Twitter on Facebook on LinkedIn on Reddit on Tumblr by Email

    Thumbnail
    View/Open
    rayner_bsc.pdf
    Metadata
     Expand record
    This service is managed by Learning, Teaching and Library
    • Archive Policy
    • Copyright and Reuse
    • Deposit Guidelines and FAQ
    • Contact Us
     

     

    Browse

    All of Research@LincolnCommunities & CollectionsTitlesAuthorsKeywordsBy Issue DateThis CollectionTitlesAuthorsKeywordsBy Issue Date

    My Account

    LoginRegister

    Statistics

    View Usage Statistics
    This service is managed by Learning, Teaching and Library
    • Archive Policy
    • Copyright and Reuse
    • Deposit Guidelines and FAQ
    • Contact Us