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    The bacteriolytic enzymes of Gliomastix murorum var. Felina

    Rhodes, Lesley
    Abstract
    Extracellular bacteriolytic activity was obtained when Gliomastix murorum var. felina was grown on heat-killed Bacillus subtilis cells (2.5 mg ml⁻¹) suspended in a defined salts solution, buffered at pH5 or at pH7. Maximal yields were measured when shake flask cultures were incubated in light at 30°C for 10 days. Lytic supernatants were designated E₁ and E₃ (fungus grown at pH5, supernatant optimally active at pH3.6 and pH8.0 respectively) and E₂ (fungus grown at pH7.0, supernatant optimally active at pH7.5). E₁ was investigated further and found to be inducible and to be repressed by glucose addition. E₁ was purified by ammonium sulphate precipitation and gel filtration on a Sephadex G-75 column. The pH optimum was 3.4 (ionic strength 0.051 and the molecular weight was estimated by gel filtration as 17000. The mode of action of the bacteriolytic enzyme was that of a β-N-acetylmuramidase. Specific activity was increased 7-fold (from 61.4 units mg⁻¹ protein to 44B units mg⁻¹ protein).... [Show full abstract]
    Keywords
    bacteriolytic enzymes; Gliomastix murorum var. felina; Bacillus subtilis; ammonium sulphate; purification; fungi; growth conditions; bacteriolytic activity; fungal biomass; glucose
    Fields of Research
    060107 Enzymes; 060101 Analytical Biochemistry; 060505 Mycology
    Date
    1987
    Type
    Thesis
    Collections
    • Masters Theses [809]
    • Department of Wine, Food and Molecular Biosciences [632]
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