Impact of biocrust on soil nitrogen maintenance and microbial composition in citrus orchards under varying urea application rates

Abstract

Biocrust communities and their effects on soil nitrogen (N) content in natural drylands have been extensively studied. However, their significance in citrus orchards, which are typically managed with N fertilizer, remains unclear. To address this, an investigation was conducted in a citrus orchard subjected to varying levels of urea application (0 %, 70 %, and 100 % of the local N fertilizer application) over three years. The study focused on biocrust communities dominated by bryophytes, examining their community and individual characteristics, and comparing the properties and microbial communities of soils with and without biocrust cover. The results showed that biocrust had the highest percent coverage (56.1 %) in the plot with 70 % local N fertilizer application. Dicranella heteromalla was the dominant biocrust species across all treatments, exhibiting greener and longer leaves with 70 % local N fertilizer application. Biocrust cover significantly (P < 0.01) influenced soil total nitrogen content, soil water content, soil total carbon content, and soil dissolved organic carbon content. In contrast, fertilizer application had non-significant effects on the physicochemical properties of soils, regardless of biocrust cover. A notable difference in bacterial phyla composition was observed between soils with and without biocrust cover (PERMANOVA, P = 0.001), although fertilization did not significantly affect the bacterial diversity index. Additionally, biocrust cover significantly (P < 0.01) influenced predicted soil bacterial functional groups, including ureolysis and nitrogen fixation, while fertilizer application had a weaker impact on these bacterial functional groups. In conclusion, nitrogen application altered the community and dominant species characteristics of biocrust but did not overshadow the effects of biocrust on soil N dynamics. In the citrus orchard with varying levels of urea application, biocrust played a crucial role in soil water retention, N maintenance, and predicted microbial ecological functions related to N cycling.