A sensitive and validated immunomagnetic-bead based enzyme-linked immunosorbent assay for analyzing total T-2 (free and modified) toxins in shrimp tissues
dc.contributor.author | Deng, Q | |
dc.contributor.author | Qiu, M | |
dc.contributor.author | Wang, Yaling, | |
dc.contributor.author | Lv, P | |
dc.contributor.author | Wu, C | |
dc.contributor.author | Sun, L | |
dc.contributor.author | Ye, Riying, | |
dc.contributor.author | Xu, D | |
dc.contributor.author | Liu, Y | |
dc.contributor.author | Gooneratne, SR | |
dc.coverage.spatial | Netherlands | |
dc.date.accessioned | 2018-08-30T04:33:22Z | |
dc.date.available | 2017-05-22 | |
dc.date.issued | 2017-08 | |
dc.date.submitted | 2017-04-17 | |
dc.description.abstract | Accurate analyses of total T-2 (free and modified) in aquatic organisms including shrimp are important as the hazard caused by T-2 has been caught increasing attention. Therefore, acurate analysis of free T-2 especially of modified T-2 in shrimp tissues is important. A rapid, sensitive, and validated method for quantitative determination of free T-2 and modified T-2 toxin was developed using immunomagnetic-bead based enzyme-linked immunosorbent assay (IMB-ELISA). Super paramagnetic particles with a carboxyl group activated by an ester method coupled with envelope antigen 3- acetylneosolaniol- hemisuccinate - ovalbumin (3-Ac-NEOS-HS-OVA) was used to form immunomagnetic beads which could bind to T-2 skeletal structure antibodies. The conditions for magnetic bead coating of T-2 skeletal structure antibodies, and the concentrations of the polyclonal antibody and HRP-labeled goat anti-rabbit antibody were optimized. A good linear relationship with T-2 concentrations ranging from 5−75 ng/mL (R² =0.9965) was observed. The detection limit of different shrimp tissues of the IMB-ELISA ranged from 2.53 to 3.20 ng/mL. And the IC₅₀ was 63 ng/mL. The recovery varied from 86% to 99% with a standard deviation of 2.8−5.8%. The application of this method to study the distribution in tissues showed that the total T-2 concentration in hepatopancreas was 26.7 µg/kg > blood > head > muscle in the highest dose group of 12.2 mg/kg. Our research showed a combination of ELISA and immunomagnetic bead technology provide a new, convenient approach to significantly improve the accuracy and sensitivity of total T-2 measurement in shrimp tissues. | |
dc.format.extent | pp.441-447 | |
dc.format.medium | ||
dc.identifier | S0147-6513(17)30247-6 | |
dc.identifier | https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=elements_prod&SrcAuth=WosAPI&KeyUT=WOS:000403384300053&DestLinkType=FullRecord&DestApp=WOS_CPL | |
dc.identifier.citation | Deng et al. A sensitive and validated immunomagnetic-bead based enzyme-linked immunosorbent assay for analyzing total T-2 (free and modified) toxins in shrimp tissues. Ecotoxicology and Environmental Safety, 142, 441-447. doi:10.1016/j.ecoenv.2017.04.037 | |
dc.identifier.doi | 10.1016/j.ecoenv.2017.04.037 | |
dc.identifier.eissn | 1090-2414 | |
dc.identifier.issn | 0147-6513 | |
dc.identifier.other | 28458227 (pubmed) | |
dc.identifier.uri | https://hdl.handle.net/10182/10192 | |
dc.language | en | |
dc.language.iso | en | |
dc.publisher | Elsevier | |
dc.relation | The original publication is available from Elsevier - https://doi.org/10.1016/j.ecoenv.2017.04.037 - http://dx.doi.org/10.1016/j.ecoenv.2017.04.037 | |
dc.relation.isPartOf | Ecotoxicology and Environmental Safety | |
dc.relation.uri | https://doi.org/10.1016/j.ecoenv.2017.04.037 | |
dc.rights | © 2017 Elsevier Inc. All rights reserved. | |
dc.subject | T-2 toxins | |
dc.subject | immunomagnetic | |
dc.subject | ELISA | |
dc.subject | shrimp tissues | |
dc.subject.anzsrc2020 | ANZSRC::4011 Environmental engineering | |
dc.subject.anzsrc2020 | ANZSRC::4102 Ecological applications | |
dc.subject.anzsrc2020 | ANZSRC::4202 Epidemiology | |
dc.subject.mesh | Animals | |
dc.subject.mesh | Penaeidae | |
dc.subject.mesh | T-2 Toxin | |
dc.subject.mesh | Enzyme-Linked Immunosorbent Assay | |
dc.subject.mesh | Immunomagnetic Separation | |
dc.subject.mesh | Reproducibility of Results | |
dc.subject.mesh | Food Contamination | |
dc.subject.mesh | Tissue Distribution | |
dc.subject.mesh | Seafood | |
dc.subject.mesh | Limit of Detection | |
dc.title | A sensitive and validated immunomagnetic-bead based enzyme-linked immunosorbent assay for analyzing total T-2 (free and modified) toxins in shrimp tissues | |
dc.type | Journal Article | |
lu.contributor.unit | LU | |
lu.contributor.unit | LU|Agriculture and Life Sciences | |
lu.contributor.unit | LU|Agriculture and Life Sciences|WFMB | |
lu.contributor.unit | LU|Research Management Office | |
lu.contributor.unit | LU|Research Management Office|OLD QE18 | |
lu.identifier.orcid | 0000-0002-5406-2894 | |
pubs.publication-status | Published | |
pubs.publisher-url | http://dx.doi.org/10.1016/j.ecoenv.2017.04.037 | |
pubs.volume | 142 |