Increased vibrissa growth in transgenic mice expressing insulin-like growth factor 1
dc.contributor.author | Su, H-Y | |
dc.contributor.author | Hickford, Jonathan | |
dc.contributor.author | The, PHB | |
dc.contributor.author | Hill, AM | |
dc.contributor.author | Frampton, CM | |
dc.contributor.author | Bickerstaffe, R | |
dc.coverage.spatial | United States | |
dc.date.accessioned | 2018-08-16T22:28:52Z | |
dc.date.available | 2015-12-08 | |
dc.date.issued | 1999-02 | |
dc.date.submitted | 1998-10-15 | |
dc.description.abstract | Insulin-like growth factor 1 (IGF-1) mediates many of the actions of growth hormone. Overexpression of IGF-1 has been reported to have endocrine and paracrine/autocrine effects on somatic growth in transgenic mice. To study the paracrine/autocrine effects of IGF-1 in hair follicles, transgenic mice were produced by pronuclear microinjection of a construct containing a mouse ultra-high sulfur keratin (UHS-KER) promoter linked to an ovine IGF-1 cDNA. This UHS-KER promoter has previously been shown to direct expression of a reporter gene to the hair follicles of transgenic mice. Four transgenic mouse lines were established as a result of microinjection of 435 embryos. Transgene expression was found in skin at day 8 and day 15 of age in three of the lines. Progeny tests were carried out by mating two of the transgenic expressing males to nontransgenic females. Mice from one line were all nonexpressors while four of the 12 mice from the other showed integration of the transgene and three expressed transgene IGF-1 mRNA in the skin. Vibrissa growth at 11-21 d of age was significantly greater in transgenic expressors than in their nontransgenic littermates. Specifically, the increase in vibrissa length for transgenics at days 11-16 (20.5%) is ≃2-fold compared with days 16-21 (11.9%). These results demonstrate that local overexpression of IGF-1 in transgenic mice is capable of stimulating vibrissa growth during the first neonatal hair cycle. | |
dc.description.sponsorship | The work was supported in part by the University Research Trust of Lincoln University. | |
dc.format.extent | pp.245-248 | |
dc.format.medium | ||
dc.identifier | S0022-202X(15)40405-1 | |
dc.identifier | https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=elements_prod&SrcAuth=WosAPI&KeyUT=WOS:000078287000017&DestLinkType=FullRecord&DestApp=WOS_CPL | |
dc.identifier.doi | 10.1046/j.1523-1747.1999.00489.x | |
dc.identifier.eissn | 1523-1747 | |
dc.identifier.issn | 0022-202X | |
dc.identifier.other | 9989803 (pubmed) | |
dc.identifier.uri | https://hdl.handle.net/10182/10146 | |
dc.language | en | |
dc.language.iso | en | |
dc.publisher | Elsevier on behalf of the Society for Investigative Dermatology, Inc. | |
dc.relation | The original publication is available from Elsevier on behalf of the Society for Investigative Dermatology, Inc. - https://doi.org/10.1046/j.1523-1747.1999.00489.x - http://dx.doi.org/10.1046/j.1523-1747.1999.00489.x | |
dc.relation.isPartOf | Journal of Investigative Dermatology | |
dc.relation.uri | https://doi.org/10.1046/j.1523-1747.1999.00489.x | |
dc.rights | © 1999 by The Society for Investigative Dermatology, Inc. All rights reserved. | |
dc.subject | insulin-like growth factor 1 (IGF-1) | |
dc.subject | transgenic mice | |
dc.subject | endocrine | |
dc.subject | paracrine | |
dc.subject | neonatal hair cycle | |
dc.subject | vibrissa growth | |
dc.subject | autocrine | |
dc.subject.anzsrc | ANZSRC::110107 Metabolic Medicine | |
dc.subject.anzsrc | ANZSRC::11 Medical and Health Sciences | |
dc.subject.anzsrc | ANZSRC::110304 Dermatology | |
dc.subject.anzsrc2020 | ANZSRC::3202 Clinical sciences | |
dc.subject.mesh | Vibrissae | |
dc.subject.mesh | Animals | |
dc.subject.mesh | Mice, Transgenic | |
dc.subject.mesh | Mice | |
dc.subject.mesh | Body Weight | |
dc.subject.mesh | Cystine | |
dc.subject.mesh | Sulfur Radioisotopes | |
dc.subject.mesh | Insulin-Like Growth Factor I | |
dc.subject.mesh | RNA, Messenger | |
dc.subject.mesh | Blotting, Southern | |
dc.subject.mesh | Gene Expression | |
dc.title | Increased vibrissa growth in transgenic mice expressing insulin-like growth factor 1 | |
dc.type | Journal Article | |
lu.contributor.unit | LU | |
lu.contributor.unit | LU|Agriculture and Life Sciences | |
lu.contributor.unit | LU|Agriculture and Life Sciences|AGSC | |
lu.contributor.unit | LU|Agriculture and Life Sciences|WFMB | |
lu.contributor.unit | LU|Research Management Office | |
lu.contributor.unit | LU|Research Management Office|OLD QE18 | |
lu.contributor.unit | LU|Centre of Excellence for One Biosecurity Research, Analysis and Synthesis | |
lu.identifier.orcid | 0000-0001-9313-761X | |
pubs.issue | 2 | |
pubs.publication-status | Published | |
pubs.publisher-url | http://dx.doi.org/10.1046/j.1523-1747.1999.00489.x | |
pubs.volume | 112 |