In vitro synthesis of a type A trichothecenes complete antigen from T-2 toxin
dc.contributor.author | Lu, P | |
dc.contributor.author | Wang, Y | |
dc.contributor.author | Wang, Y | |
dc.contributor.author | Wu, C | |
dc.contributor.author | Sun, L | |
dc.contributor.author | Xu, D | |
dc.contributor.author | Sun, X | |
dc.contributor.author | Li, J | |
dc.contributor.author | Gooneratne, R | |
dc.date.accessioned | 2020-06-23T04:09:28Z | |
dc.date.available | 2016-07-27 | |
dc.date.issued | 2016-11-01 | |
dc.date.submitted | 2016-05-22 | |
dc.description.abstract | We report the artificial synthesis from T-2 toxin of a type A trichothecenes complete antigen. First, 3-Ac-T-2 was made from T-2 following acetylation. Then 3-Ac-NEOS as a T-2 skeleton structure was synthesized by enzymolysis and identified by liquid chromatography-tandem mass spectrometry and nuclear magnetic resonance. Next, a hapten (3-Ac-NEOS-HS) was formed by modifying the C8 position of 3-Ac-NEOS using succinic anhydride method. 3-Ac-NEOS-HS-BSA/-OVA were prepared by conjugating hapten with bovine serum albumin (BSA) or ovalbumin (OVA) by carbodiimide method and identified by UV spectroscopy and sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Results showed that conjugation ratio of 3-Ac-NEOS-HS to BSA was 8.76: 1 and OVA 7.24: 1, indicating 3-Ac-NEOS-HS-BSA as a complete antigen was better. Next we used it to immunize rabbits and obtained a 1:64,000 antibody titre. In conclusion, a type A trichothecenes complete antigen was successfully synthesized, which was the foundation for antibody preparation and enzyme-linked immunosorbent assay kit development for all type A trichothecenes (parent + modified/masked type A trichothecenes). | |
dc.format.extent | pp.870-885 | |
dc.identifier | https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=elements_prod&SrcAuth=WosAPI&KeyUT=WOS:000383965200011&DestLinkType=FullRecord&DestApp=WOS_CPL | |
dc.identifier.doi | 10.1080/09540105.2016.1202205 | |
dc.identifier.eissn | 1465-3443 | |
dc.identifier.issn | 0954-0105 | |
dc.identifier.other | DW9GS (isidoc) | |
dc.identifier.uri | https://hdl.handle.net/10182/12119 | |
dc.language | en | |
dc.language.iso | en | |
dc.publisher | Taylor & Francis | |
dc.relation | The original publication is available from Taylor & Francis - https://doi.org/10.1080/09540105.2016.1202205 - http://dx.doi.org/10.1080/09540105.2016.1202205 | |
dc.relation.isPartOf | Food and Agricultural Immunology | |
dc.relation.uri | https://doi.org/10.1080/09540105.2016.1202205 | |
dc.rights | © 2016 Informa UK Limited, trading as Taylor & Francis Group | |
dc.subject | Type A trichotehcenes | |
dc.subject | T-2 toxin | |
dc.subject | 3-Ac-NEOS | |
dc.subject | skeleton | |
dc.subject | complete antigen | |
dc.subject.anzsrc2020 | ANZSRC::3006 Food sciences | |
dc.subject.anzsrc2020 | ANZSRC::3204 Immunology | |
dc.title | In vitro synthesis of a type A trichothecenes complete antigen from T-2 toxin | |
dc.type | Journal Article | |
lu.contributor.unit | LU | |
lu.contributor.unit | LU|Agriculture and Life Sciences | |
lu.contributor.unit | LU|Agriculture and Life Sciences|WFMB | |
lu.contributor.unit | LU|Research Management Office | |
lu.contributor.unit | LU|Research Management Office|OLD QE18 | |
lu.identifier.orcid | 0000-0002-5406-2894 | |
pubs.issue | 6 | |
pubs.publication-status | Published | |
pubs.publisher-url | http://dx.doi.org/10.1080/09540105.2016.1202205 | |
pubs.volume | 27 |