hemX is required for production of 2-ketogluconate, the predominant organic anion required for inorganic phosphate solubilization by Burkholderia sp. Ha185
dc.contributor.author | Hsu, LPC | |
dc.contributor.author | Condron, L | |
dc.contributor.author | O'Callaghan, M | |
dc.contributor.author | Hurst, MRH | |
dc.coverage.spatial | United States | |
dc.date.accessioned | 2018-05-14T02:07:11Z | |
dc.date.available | 2015-08-10 | |
dc.date.issued | 2015-12 | |
dc.date.submitted | 2015-08-04 | |
dc.description.abstract | The bacterium Burkholderia sp. Ha185 readily solubilizes inorganic phosphate by releasing the low molecular weight organic anion, 2-ketogluconate. Using random transposon mutagenesis and in silico analysis, a mutation that caused almost complete abolition of phosphate solubilization was located within hemX, which is part of the hem operon. Burkholderia sp. Ha185 HemX is a multidomain protein, predicted to encode a bifunctional uroporphyrinogen-III synthetase/uroporphyrin-III C-methyltransferase, which has not previously been implicated in phosphate solubilization. Complementation of hemX restored the ability of the mutant to solubilize phosphate in both plate and liquid cultures. Based on a combination of organic-anion profiling, quantitative polymerase chain reaction and in silico analyses, hemX was confirmed to be solely responsible for hydroxyapatite solubilization in Burkholderia sp. Ha185. It is proposed that the biosynthesis of a yet to be determined redox cofactor by HemX is the main pathway for generating 2-ketogluconate via a haem-dependent gluconate 2-dehydrogenase in Burkholderia sp. Ha185. | |
dc.format.extent | pp.918-928 | |
dc.format.medium | Print-Electronic | |
dc.identifier | https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=elements_prod&SrcAuth=WosAPI&KeyUT=WOS:000368136100013&DestLinkType=FullRecord&DestApp=WOS_CPL | |
dc.identifier.citation | Hsu, P. C. L., Condron, L., O'callaghan, M., & Hurst, M. R. (2015). hemX is required for production of 2‐ketogluconate, the predominant organic anion required for inorganic phosphate solubilization by Burkholderia sp. Ha185. Environmental microbiology reports, 7(6), 918-928. | |
dc.identifier.doi | 10.1111/1758-2229.12326 | |
dc.identifier.eissn | 1758-2229 | |
dc.identifier.issn | 1758-2229 | |
dc.identifier.other | 26256849 (pubmed) | |
dc.identifier.uri | https://hdl.handle.net/10182/9361 | |
dc.language | eng | |
dc.language.iso | eng | |
dc.publisher | Wiley on behalf of the Society for Applied Microbiology | |
dc.relation | The original publication is available from Wiley on behalf of the Society for Applied Microbiology - https://doi.org/10.1111/1758-2229.12326 - http://dx.doi.org/10.1111/1758-2229.12326 | |
dc.relation.isPartOf | Environmental Microbiology Reports | |
dc.relation.uri | https://doi.org/10.1111/1758-2229.12326 | |
dc.rights | © 2015 Society for Applied Microbiology and John Wiley & Sons Ltd | |
dc.subject | Burkholderia sp. Ha 185 | |
dc.subject | transposon mutagenesis | |
dc.subject | hemX | |
dc.subject | multi-domain protein | |
dc.subject | phosphate solubilization | |
dc.subject | silico analyses | |
dc.subject.anzsrc2020 | ANZSRC::3103 Ecology | |
dc.subject.anzsrc2020 | ANZSRC::3104 Evolutionary biology | |
dc.subject.anzsrc2020 | ANZSRC::3107 Microbiology | |
dc.subject.mesh | Burkholderia | |
dc.subject.mesh | Phosphates | |
dc.subject.mesh | Gluconates | |
dc.subject.mesh | Hydroxymethylbilane Synthase | |
dc.subject.mesh | Carbohydrate Dehydrogenases | |
dc.subject.mesh | Gene Expression Regulation, Bacterial | |
dc.subject.mesh | Gene Order | |
dc.subject.mesh | Mutation | |
dc.subject.mesh | Operon | |
dc.subject.mesh | Solubility | |
dc.subject.mesh | Models, Biological | |
dc.subject.mesh | Metabolic Networks and Pathways | |
dc.title | hemX is required for production of 2-ketogluconate, the predominant organic anion required for inorganic phosphate solubilization by Burkholderia sp. Ha185 | |
dc.type | Journal Article | |
lu.contributor.unit | LU | |
lu.contributor.unit | LU|Agriculture and Life Sciences | |
lu.contributor.unit | LU|Agriculture and Life Sciences|SOILS | |
lu.contributor.unit | LU|Research Management Office | |
lu.contributor.unit | LU|Research Management Office|OLD QE18 | |
lu.identifier.orcid | 0000-0001-9989-630X | |
lu.identifier.orcid | 0000-0002-3082-994X | |
pubs.issue | 6 | |
pubs.publication-status | Published | |
pubs.publisher-url | http://dx.doi.org/10.1111/1758-2229.12326 | |
pubs.volume | 7 |