A sensitive and validated method for determination of T-2 and HT-2 toxin residues in shrimp tissues by LC-MS/MS
dc.contributor.author | Lu, P | |
dc.contributor.author | Wu, C | |
dc.contributor.author | Shi, Q | |
dc.contributor.author | Wang, Yaling, | |
dc.contributor.author | Sun, L | |
dc.contributor.author | Liao, J | |
dc.contributor.author | Zhong, S | |
dc.contributor.author | Xu, D | |
dc.contributor.author | Chen, J | |
dc.contributor.author | Liu, Y | |
dc.contributor.author | Li, J | |
dc.contributor.author | Gooneratne, SR | |
dc.date.accessioned | 2016-10-05T22:44:29Z | |
dc.date.available | 2015-10-20 | |
dc.date.issued | 2016-06 | |
dc.date.submitted | 2015-10-11 | |
dc.description.abstract | Accurate analyses of T-2 and HT-2 toxin in aquatic organisms including shrimp are important as these two toxins are increasingly detected in aquatic cereal-based feed. Therefore, the potential for these toxins to enter the human food chain from contaminated fish and shrimp products is very real. A rapid, sensitive, and validated method for simultaneous determination of T-2 and HT-2 toxins was developed using a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method following extraction of the two toxins from shrimp tissues with ethyl acetate. This method is simple in that additional solid-phase extraction is not required to isolate and purify the toxins. LC was performed on an analytical Hypersil GOLD column. The mobile phase consisted of methanol and 5 mM ammonium acetate containing 0.1 % formic acid. The MS/MS ion transitions for both the T-2 toxin (484.20 → 214.87) and HT-2 toxin (442.20 → 214.96) were monitored. And the most intense transition ion product (m/z) of T-2 and HT-2 used for quantification on the SRM mode of a mass spectrometer was 304.95 and 262.91, respectively. The results linearly correlated with coefficients >0.9990. The limits of quantification ranged from 0.02 to 0.51 ng·g−1 and from 0.17 to 4.48 ng·g−1 for T-2 and HT-2, respectively, depending on the shrimp tissue type. The overall extraction recovery for both toxins ranged between 84 and 111 % with RSD values less than 15.0 %, indicative of good replication. Furthermore, the recovery and precision levels were within the predefined limits (≤15 %) at all concentrations. The application of this method to study the accumulation of T-2 toxin in shrimp showed that it can be successfully used to monitor even very low tissue toxin concentrations. Research is in progress to extend this method for the measurement of T-2 and HT-2 in aquatic foods that enter the human food chain. | |
dc.format.extent | pp.1580-1594 | |
dc.identifier | https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=elements_prod&SrcAuth=WosAPI&KeyUT=WOS:000375331900013&DestLinkType=FullRecord&DestApp=WOS_CPL | |
dc.identifier.citation | Lu, P., Wu, C., Shi, Q., Wang, Y., Sun, L., Liao, J., . . . Gooneratne, R. (2016). A Sensitive and Validated Method for Determination of T-2 and HT-2 Toxin Residues in Shrimp Tissues by LC-MS/MS. Food Analytical Methods, 9(6), 1580-1594. | |
dc.identifier.doi | 10.1007/s12161-015-0336-y | |
dc.identifier.eissn | 1936-976X | |
dc.identifier.issn | 1936-9751 | |
dc.identifier.other | DL0OI (isidoc) | |
dc.identifier.uri | https://hdl.handle.net/10182/7428 | |
dc.language | English | |
dc.language.iso | English | |
dc.publisher | Springer US | |
dc.relation | The original publication is available from Springer US - https://doi.org/10.1007/s12161-015-0336-y - http://dx.doi.org/10.1007/s12161-015-0336-y | |
dc.relation.isPartOf | Food Analytical Methods | |
dc.relation.uri | https://doi.org/10.1007/s12161-015-0336-y | |
dc.rights | © Springer Science+Business Media New York 2015 | |
dc.rights.ccname | Publisher's own licence | |
dc.rights.ccuri | Publisher's own licence | |
dc.rights.licence | Publisher's own licence | |
dc.subject | T-2 toxin | |
dc.subject | HT-2 toxin | |
dc.subject | LC-MS/MS | |
dc.subject | shrimp | |
dc.subject | method validation | |
dc.subject.anzsrc2020 | ANZSRC::3006 Food sciences | |
dc.title | A sensitive and validated method for determination of T-2 and HT-2 toxin residues in shrimp tissues by LC-MS/MS | |
dc.type | Journal Article | |
lu.contributor.unit | LU | |
lu.contributor.unit | LU|Agriculture and Life Sciences | |
lu.contributor.unit | LU|Agriculture and Life Sciences|WFMB | |
lu.contributor.unit | LU|Research Management Office | |
lu.contributor.unit | LU|Research Management Office|OLD QE18 | |
lu.identifier.orcid | 0000-0002-5406-2894 | |
pubs.issue | 6 | |
pubs.publication-status | Published | |
pubs.publisher-url | http://dx.doi.org/10.1007/s12161-015-0336-y | |
pubs.volume | 9 |