Associations between variation in the ovine high glycine-tyrosine keratin-associated protein gene KRTAP20-1 and wool traits
dc.contributor.author | Gong, H | |
dc.contributor.author | Zhou, Huitong | |
dc.contributor.author | Bai, Lingrong, | |
dc.contributor.author | Li, W | |
dc.contributor.author | Li, S | |
dc.contributor.author | Wang, J | |
dc.contributor.author | Luo, Y | |
dc.contributor.author | Hickford, Jonathan | |
dc.coverage.spatial | United States | |
dc.date.accessioned | 2019-07-03T00:59:12Z | |
dc.date.available | 2018-12-08 | |
dc.date.issued | 2019-02 | |
dc.date.submitted | 2018-12-06 | |
dc.description.abstract | The keratin-associated proteins (KAPs) are important constituents of wool fibres. Of the many mammalian KAP genes (KRTAPs) identified, KRTAP20-1 has been described in humans, but it has not been described in any other species. A search of the sheep genome using the human KRTAP20-1 sequence revealed a homologous open reading frame on chromosome 1, which would encode a high glycine-tyrosine KAP. PCR-single stranded conformational polymorphism (PCR-SSCP) analysis identified eight different banding patterns representing eight unique DNA sequences (named A-H). The sequences had highest similarity to the human KRTAP20-1 sequence, and this suggests they are variants of ovine KRTAP20-1. Among these variants, a 12-bp insertion/deletion and six single nucleotide polymorphisms (SNPs) including one 5' untranslated region (UTR) SNP, one 3' UTR SNP and two non-synonymous SNPs, were detected. Variant A was found to be associated with a decrease in mean fibre diameter, fibre diameter standard deviation and prickle factor; while variant C was associated with increased greasy fleece weight and decreased wool yield. These associations persisted after adjusting for the effect of two nearby KRTAPs (KRTAP6-3 and KRTAP22-1) that have also been reported to associate with these wool traits. This suggests that variation in KRTAP20-1 affects wool yield and mean fibre diameter-associated traits, and that this effect is unlikely to be the result of the clustering of these KRTAPs on chromosome 1. | |
dc.format.extent | pp.587-595 | |
dc.format.medium | ||
dc.identifier | 5235606 | |
dc.identifier | https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=elements_prod&SrcAuth=WosAPI&KeyUT=WOS:000457478200008&DestLinkType=FullRecord&DestApp=WOS_CPL | |
dc.identifier.doi | 10.1093/jas/sky465 | |
dc.identifier.eissn | 1525-3163 | |
dc.identifier.issn | 0021-8812 | |
dc.identifier.other | 30535023 (pubmed) | |
dc.identifier.uri | https://hdl.handle.net/10182/10807 | |
dc.language | eng | |
dc.language.iso | eng | |
dc.publisher | Oxford University Press on behalf of American Society of Animal Science | |
dc.relation | The original publication is available from Oxford University Press on behalf of American Society of Animal Science - https://doi.org/10.1093/jas/sky465 - http://dx.doi.org/10.1093/jas/sky465 | |
dc.relation.isPartOf | Journal of Animal Science | |
dc.relation.uri | https://doi.org/10.1093/jas/sky465 | |
dc.rights | © The Author(s) 2018. Published by Oxford University Press on behalf of the American Society of Animal Science. All rights reserved. | |
dc.subject | keratin-associated protein KAP20-1 gene ( KRTAP20-1 ) | |
dc.subject | mean fiber diameter | |
dc.subject | sheep | |
dc.subject | variation | |
dc.subject | wool yield | |
dc.subject.anzsrc2020 | ANZSRC::30 Agricultural, veterinary and food sciences | |
dc.subject.anzsrc2020 | ANZSRC::31 Biological sciences | |
dc.subject.mesh | Wool | |
dc.subject.mesh | Animals | |
dc.subject.mesh | Sheep | |
dc.subject.mesh | Tyrosine | |
dc.subject.mesh | Glycine | |
dc.subject.mesh | Polymerase Chain Reaction | |
dc.subject.mesh | Sequence Alignment | |
dc.subject.mesh | Phylogeny | |
dc.subject.mesh | Base Sequence | |
dc.subject.mesh | Phenotype | |
dc.subject.mesh | Polymorphism, Single-Stranded Conformational | |
dc.subject.mesh | Polymorphism, Single Nucleotide | |
dc.subject.mesh | Genome | |
dc.subject.mesh | Keratins | |
dc.title | Associations between variation in the ovine high glycine-tyrosine keratin-associated protein gene KRTAP20-1 and wool traits | |
dc.type | Journal Article | |
lu.contributor.unit | LU | |
lu.contributor.unit | LU|Agriculture and Life Sciences | |
lu.contributor.unit | LU|Agriculture and Life Sciences|AGSC | |
lu.contributor.unit | LU|Research Management Office | |
lu.contributor.unit | LU|Research Management Office|OLD QE18 | |
lu.contributor.unit | LU|Centre of Excellence for One Biosecurity Research, Analysis and Synthesis | |
lu.identifier.orcid | 0000-0001-7620-3160 | |
lu.identifier.orcid | 0000-0001-9313-761X | |
pubs.issue | 2 | |
pubs.publication-status | Published | |
pubs.publisher-url | http://dx.doi.org/10.1093/jas/sky465 | |
pubs.volume | 97 |