Cytotoxicity of T-2 and modified T-2 toxins: Induction of JAK/STAT pathway in RAW264.7 cells by hepatopancreas and muscle extracts of shrimp fed with T-2 toxin
dc.contributor.author | Wang, Xing, | |
dc.contributor.author | Wang, Yaling, | |
dc.contributor.author | Qiu, M | |
dc.contributor.author | Sun, L | |
dc.contributor.author | Wang, X | |
dc.contributor.author | Li, Caihong, | |
dc.contributor.author | Xu, D | |
dc.contributor.author | Gooneratne, SR | |
dc.coverage.spatial | England | |
dc.date.accessioned | 2018-05-21T23:28:00Z | |
dc.date.available | 2017-01-09 | |
dc.date.issued | 2017-03-01 | |
dc.date.submitted | 2017-01-09 | |
dc.description.abstract | T-2 can be biotransformed in animal tissues to modified T-2s (mT-2s). Food contaminated with T-2 and/or mT-2s is a hazard to both animals and humans, including the immune system. In this study, Litopenaeus vannamei were fed T-2 orally for 20 d, and hepatopancreas and muscle extracts, T-2, and T-2-glucuronide (T-2-GluA) were added to RAW264.7 in vitro and their effects on the JAK/STAT pathway were examined. STAT2 mRNA gene expression induced by hepatopancreas and muscle extracts was markedly higher compared with that of T-2 or T-2-GluA group. SCOSs, IL-6 and IL-1β mRNA gene expressions induced by hepatopancreas extract were greater than those induced by muscle extract. Muscle extract significantly activated STAT3 phosphorylation but inhibited STAT1 phosphorylation. Activation of the JAK/STAT pathway by hepatopancreas mT-2s was significantly higher than that by muscle extracts. Muscle and hepatopancreas extracts and T-2 also significantly induced IL-6 mRNA gene expression. With reference to phosphorylation levels, significant activation of JAK1 and STAT2 occurred with T-2 and JAK3 by muscle extract, JAK2 by hepatopancreas extract and STAT1 by T-2-GluA. This study showed that both T-2 and mT-2s are cytotoxic but the activation of the JAK/STAT pathway in RAW264.7 cells by T-2 was greater than that by mT-2s in hepatopancreas and muscle extracts from T-2-fed Litopenaeus vannamei. | |
dc.format.extent | pp.144-151 | |
dc.format.medium | Electronic-eCollection | |
dc.identifier | c6tx00392c | |
dc.identifier | https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=elements_prod&SrcAuth=WosAPI&KeyUT=WOS:000396138500003&DestLinkType=FullRecord&DestApp=WOS_CPL | |
dc.identifier.doi | 10.1039/c6tx00392c | |
dc.identifier.eissn | 2045-452X | |
dc.identifier.issn | 2045-4538 | |
dc.identifier.other | EN6UI (isidoc) | |
dc.identifier.other | 30090484 (pubmed) | |
dc.identifier.uri | https://hdl.handle.net/10182/9420 | |
dc.language | en | |
dc.language.iso | en | |
dc.publisher | The Royal Society of Chemistry | |
dc.relation | The original publication is available from The Royal Society of Chemistry - https://doi.org/10.1039/c6tx00392c - http://dx.doi.org/10.1039/c6tx00392c | |
dc.relation.isPartOf | Toxicology Research | |
dc.relation.uri | https://doi.org/10.1039/c6tx00392c | |
dc.rights | © The Royal Society of Chemistry 2017 | |
dc.subject | cytotoxicity | |
dc.subject | T-2 toxins | |
dc.subject | RAW264.7 cells | |
dc.subject | JAK/STAT pathway | |
dc.subject | Litopenaeus vannamei | |
dc.subject | shrimp | |
dc.subject.anzsrc | ANZSRC::111506 Toxicology (incl. Clinical Toxicology) | |
dc.subject.anzsrc2020 | ANZSRC::3214 Pharmacology and pharmaceutical sciences | |
dc.title | Cytotoxicity of T-2 and modified T-2 toxins: Induction of JAK/STAT pathway in RAW264.7 cells by hepatopancreas and muscle extracts of shrimp fed with T-2 toxin | |
dc.type | Journal Article | |
lu.contributor.unit | LU | |
lu.contributor.unit | LU|Agriculture and Life Sciences | |
lu.contributor.unit | LU|Agriculture and Life Sciences|WFMB | |
lu.contributor.unit | LU|Research Management Office | |
lu.contributor.unit | LU|Research Management Office|OLD QE18 | |
lu.identifier.orcid | 0000-0002-5406-2894 | |
pubs.issue | 2 | |
pubs.publication-status | Published | |
pubs.publisher-url | http://dx.doi.org/10.1039/c6tx00392c | |
pubs.volume | 6 |