Haplotyping using a combination of polymerase chain reaction–single-strand conformational polymorphism analysis and haplotype-specific PCR amplification
dc.contributor.author | Zhou, Huitong | |
dc.contributor.author | Li, S | |
dc.contributor.author | Liu, X | |
dc.contributor.author | Wang, J | |
dc.contributor.author | Luo, Y | |
dc.contributor.author | Hickford, Jonathan | |
dc.coverage.spatial | United States | |
dc.date.accessioned | 2018-01-11T21:56:54Z | |
dc.date.available | 2014-08-28 | |
dc.date.issued | 2014-12-01 | |
dc.date.submitted | 2014-08-20 | |
dc.description.abstract | A single nucleotide polymorphism (SNP) may have an impact on phenotype, but it may also be influenced by multiple SNPs within a gene; hence, the haplotype or phase of multiple SNPs needs to be known. Various methods for haplotyping SNPs have been proposed, but a simple and cost-effective method is currently unavailable. Here we describe a haplotyping approach using two simple techniques: polymerase chain reaction–single-strand conformational polymorphism (PCR–SSCP) and haplotype-specific PCR. In this approach, individual regions of a gene are analyzed by PCR–SSCP to identify variation that defines sub-haplotypes, and then extended haplotypes are assembled from the sub-haplotypes either directly or with the additional use of haplotype-specific PCR amplification. We demonstrate the utility of this approach by haplotyping ovine FABP4 across two variable regions that contain seven SNPs and one indel. The simplicity of this approach makes it suitable for large-scale studies and/or diagnostic screening. | |
dc.format.extent | pp.59-64 | |
dc.format.medium | Print-Electronic | |
dc.identifier | S0003-2697(14)00376-5 | |
dc.identifier | https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=elements_prod&SrcAuth=WosAPI&KeyUT=WOS:000344445200012&DestLinkType=FullRecord&DestApp=WOS_CPL | |
dc.identifier.citation | Zhou et al. (2014). Haplotyping using a combination of polymerase chain reaction–single-strand conformational polymorphism analysis and haplotype-specific PCR amplification. Analytical Biochemistry, 466, 59-64. doi:10.1016/j.ab.2014.08.021 | |
dc.identifier.doi | 10.1016/j.ab.2014.08.021 | |
dc.identifier.eissn | 1096-0309 | |
dc.identifier.issn | 0003-2697 | |
dc.identifier.other | 25173511 (pubmed) | |
dc.identifier.uri | https://hdl.handle.net/10182/8871 | |
dc.language | en | |
dc.language.iso | en | |
dc.publisher | Elsevier | |
dc.relation | The original publication is available from Elsevier - https://doi.org/10.1016/j.ab.2014.08.021 - http://dx.doi.org/10.1016/j.ab.2014.08.021 | |
dc.relation.isPartOf | Analytical Biochemistry | |
dc.relation.uri | https://doi.org/10.1016/j.ab.2014.08.021 | |
dc.rights | © 2014 Elsevier Inc. All rights reserved. | |
dc.subject | haplotyping | |
dc.subject | SNP haplotype | |
dc.subject | PCR–SSCP | |
dc.subject | haplotype-specific PCR amplification | |
dc.subject.anzsrc | ANZSRC::060101 Analytical Biochemistry | |
dc.subject.anzsrc2020 | ANZSRC::3101 Biochemistry and cell biology | |
dc.subject.anzsrc2020 | ANZSRC::3401 Analytical chemistry | |
dc.subject.mesh | Animals | |
dc.subject.mesh | Sheep | |
dc.subject.mesh | Humans | |
dc.subject.mesh | DNA Fingerprinting | |
dc.subject.mesh | Nucleic Acid Amplification Techniques | |
dc.subject.mesh | Polymerase Chain Reaction | |
dc.subject.mesh | Base Sequence | |
dc.subject.mesh | Haplotypes | |
dc.subject.mesh | Polymorphism, Single-Stranded Conformational | |
dc.subject.mesh | Molecular Sequence Data | |
dc.subject.mesh | Fatty Acid-Binding Proteins | |
dc.title | Haplotyping using a combination of polymerase chain reaction–single-strand conformational polymorphism analysis and haplotype-specific PCR amplification | |
dc.type | Journal Article | |
lu.contributor.unit | LU | |
lu.contributor.unit | LU|Agriculture and Life Sciences | |
lu.contributor.unit | LU|Agriculture and Life Sciences|AGSC | |
lu.contributor.unit | LU|Research Management Office | |
lu.contributor.unit | LU|Research Management Office|OLD QE18 | |
lu.contributor.unit | LU|Centre of Excellence for One Biosecurity Research, Analysis and Synthesis | |
lu.identifier.orcid | 0000-0001-7620-3160 | |
lu.identifier.orcid | 0000-0001-9313-761X | |
pubs.publication-status | Published | |
pubs.publisher-url | http://dx.doi.org/10.1016/j.ab.2014.08.021 | |
pubs.volume | 466 |